Rif1 Antibody

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: Rif1 Antibody [NB300-973] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rif1 Antibody Summary

• Description: Novus Biologicals Goat Rif1 Antibody (NB300-973) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Rif1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence CEEGIIDANKTETNT corresponding to internal region according to NP_060621.3.
• Specificity: This antibody is expected to recognise an epitope corresponding to aa 2076-2089 of human RIF1 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RIF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the predicted MW of approx. 247 kDa band according to NP_060621.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rif1 Antibody

• DKFZp434D1026
• DKFZp781N1478
• FLJ10599
• FLJ12870
• RAP1 interacting factor homolog (yeast)
• Rap1-interacting factor 1 homolog
• telomere-associated protein RIF1

Background

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rif1 Antibody (NB300-973)(1)

Publication using NB300-973

• Silverman J, Takai H, Buonomo SB et al. Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint. Genes Dev 2004-09-01 [PMID: 15342490]

Bioinformatics

• Gene Symbol: RIF1
• Entrez:
  • 55183(Human)
• Uniprot:
  • NP_060621(Human)