Rif1 Antibody

Western Blot: Rif1 Antibody [NB100-80875] - Affinity Purified anti-Rif1 antibody shows detection of a band ~265 kD corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 25 ug of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-80875] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry-Paraffin: Rif1 Antibody [NB100-80875] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

Rif1 Antibody Summary

• Immunogen: Synthetic peptide corresponding to C-Terminal region near aa 2375-2419 of Mouse Rif1.
• Epitope: C-Terminal region near aa 2375-2419
• Specificity: Databases confirm that the immunogen sequence represents the C-terminal end of mouse Rif1 (534 aa 58.1 kDa), mouse telomere-associated protein RIF1 (2418 aa 265.7 kDa) and mouse Rap1-interacting factor 1 (2426 267.1 kDa).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RIF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:40000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:500 - 1:3000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rif1 Antibody

• DKFZp434D1026
• DKFZp781N1478
• FLJ10599
• FLJ12870
• RAP1 interacting factor homolog (yeast)
• Rap1-interacting factor 1 homolog
• telomere-associated protein RIF1

Background

Rif1 (Rap1-interacting factor homolog) is a protein whose primary function is in the DNA-damage response, RIF1 forms foci that colocalize with other DNA damage response factors, after induction of double-strand breaks. Rif1 localizes to double strand breaks in an ATM (ataxia telangiectasia mutated)- and 53BP1-dependent manner and functions in the intra-S-phase checkpoint that serves to slow down DNA synthesis when DNA damage has occurred.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RIF1
• Entrez:
  • 55183(Human)
  • 51869(Mouse)
  • 295602(Rat)
• Uniprot:
  • Q6PR54(Mouse)