Rictor Antibody

Images

 
Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictoron HeLa whole cell lysate using NB100-611. Rictor was also IPed using rabbit anti-Rictor antibodies NB100-612 and another one.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Rictor Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).
Specificity
NB100-611 is specific for human Rictor protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RICTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 3 - 10 ug/mg lysate
Application Notes
Blots performed with NB 100-611 show additional bands not seen with NB 100-612.
Publications
Read Publications using NB100-611.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rictor Antibody

  • AVO3 homolog
  • AVO3
  • hAVO3
  • KIAA1999DKFZp686B11164
  • mAVO3
  • MGC39830
  • PIA
  • Pianissimo
  • rapamycin-insensitive companion of mTOR
  • Rictor
  • RPTOR independent companion of MTOR, complex 2
  • TORC2-specific protein AVO3

Background

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for Rictor Antibody (NB100-611)(33)


Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-611 Applications Species
James MF, Stivison E, Beauchamp R et al. Regulation of mTOR complex 2 signaling in neurofibromatosis 2-deficient target cell types. Mol Cancer Res 2012 May [PMID: 22426462]
Ansell SM, Tang H, Kurtin PJ et al. Temsirolimus and rituximab in patients with relapsed or refractory mantle cell lymphoma: a phase 2 study Lancet Oncol 2011 Mar 24 [PMID: 21440503]
Murata H, Sakaguchi M, Jin Y et al. A new cytosolic pathway from a Parkinson disease-associated kinase, BRPK/PINK1: activation of AKT via mTORC2. J Biol Chem 2011 Mar [PMID: 21177249]
Liu L, Luo Y, Chen L et al. Rapamycin inhibits cytoskeleton reorganization and cell motility by suppressing RhoA expression and activity. J Biol Chem 2010 Dec [PMID: 20937815]
Fu L, Kim YA, Wang X et al. Perifosine inhibits mammalian target of rapamycin signaling through facilitating degradation of major components in the mTOR axis and induces autophagy. Cancer Res 2009 Dec [PMID: 19920197]
Dibble CC, Asara JM, Manning BD et al. Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1. Mol Cell Biol 2009 Nov [PMID: 19720745]
Yu K, Toral-Barza L, Shi C et al. Biochemical, Cellular, In vivo Activity of Novel ATP-Competitive Selective Inhibitors of the Mammalian Target of Rapamycin. Cancer Res 2009 [PMID: 19584280]
Shu L, Houghton PJ. The mTORC2 complex regulates terminal differentiation of C2C12 myoblasts. Mol Cell Biol 2009 Sep [PMID: 19564418]
Breuleux M, Klopfenstein M, Stephan C, et al. Increased AKT S473 phosphorylation after mTORC1 inhibition is rictor dependent does not predict tumor cell response to PI3K/mTOR inhibition. Mol Cancer Ther 2009 [PMID: 19372546]
Sato T, Nakashima A, Guo L et al. Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein. J Biol Chem 2009 May [PMID: 19299511]
Show All 33 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Rictor Antibody (NB100-611)

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Research Areas for Rictor Antibody (NB100-611)

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Blogs on Rictor.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol RICTOR
Entrez
Uniprot