RIAM/APBB1IP Antibody

Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIAM/APBB1IP at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

RIAM/APBB1IP Antibody Summary

• Immunogen: E. coli-derived recombinant human RIAM/APBB1IP
  Gly2-Ser89
  Accession # Q7Z5R6
• Specificity: Detects recombinant human RIAM/APBB1IP in direct ELISAs. Detects human and mouse RIAM/APBB1IP in Western blots
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: APBB1IP
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIAM/APBB1IP Antibody

• amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein
• amyloid beta A4 precursor protein-binding family B member 1-interacting protein
• APBB1-interacting protein 1
• APBB1IP
• INAG1
• PREL1
• PREL-1
• proline rich EVH1 ligand 1
• Proline-rich EVH1 ligand 1
• Proline-rich protein 73
• Rap1-GTP-interacting adapter molecule
• Rap1-interacting adaptor molecule
• RARP1
• Retinoic acid-responsive proline-rich protein 1
• RIAM
• RIAMRARP-1

Background

APBB1IP (Amyloid beta A4 precursor protein-binding family B member 1 interacting protein; also PREL-1, Pro-rich protein 73, RARP1 and RIAM) is a member of the MRL family of proteins. Although its predicted MW is 73 kDa, it runs anomalously at 100-110 kDa in SDS-PAGE. It is expressed in megakaryocytes, T cells and fibroblasts. APBB1IP acts as a coordinator for Rap1 GTPase signaling. Rap1 both antagonizes Ras signaling, and is associated with beta 1 and beta 2 integrin-induced cell adhesion and cytoskeleton rearrangement. Within this context, APBB1IP operates as a cytosolic adaptor protein that induces integrin activation by recruiting talin and RAP1 to the cell membrane, and by mediating Profilin and VASP protein polymerizatiuon of actin. Human APBB1IP is 666 amino acids (aa) in length. It contains a poly-Pro sequence (aa 129-148), a Ras-association domain (aa 176-263), a PH domain (aa 310-419), and a large poly-Pro region (aa 503-640). There are multiple potential isoform variants. One contains a deletion of aa 152-177 coupled to a 27 aa substitution for aa 262-666, a second possesses a 21 aa substitution for aa 152‑666, and a third shows a deletion of aa 386-390 coupled to a five aa substitution for aa 417-666. Over aa 2-89, human APBB1IP shares 97% aa sequence identity with mouse APBB1IP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RIAM/APBB1IP Antibody (AF7279)(1)

Publication using AF7279

• F Lagarrigue, FB Gertler, MH Ginsberg, JM Cantor Cutting Edge: Loss of T Cell RIAM Precludes Conjugate Formation with APC and Prevents Immune-Mediated Diabetes J. Immunol, 2017;0(0):. 2017 [PMID: 28348273] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: APBB1IP
• Uniprot:
  • Q7Z5R6(Human)