RIAM/APBB1IP Antibody

Images

 
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified ...read more
RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

RIAM/APBB1IP Antibody Summary

Immunogen
E. coli-derived recombinant human RIAM/APBB1IP
Gly2-Ser89
Accession # Q7Z5R6
Specificity
Detects recombinant human RIAM/APBB1IP in direct ELISAs. Detects human and mouse RIAM/APBB1IP in Western blots
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
APBB1IP
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
AF7279 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RIAM/APBB1IP Antibody

  • amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein
  • amyloid beta A4 precursor protein-binding family B member 1-interacting protein
  • APBB1-interacting protein 1
  • APBB1IP
  • INAG1
  • PREL1
  • PREL-1
  • proline rich EVH1 ligand 1
  • Proline-rich EVH1 ligand 1
  • Proline-rich protein 73
  • Rap1-GTP-interacting adapter molecule
  • Rap1-interacting adaptor molecule
  • RARP1
  • Retinoic acid-responsive proline-rich protein 1
  • RIAM
  • RIAMRARP-1

Background

APBB1IP (Amyloid beta A4 precursor protein-binding family B member 1 interacting protein; also PREL-1, Pro-rich protein 73, RARP1 and RIAM) is a member of the MRL family of proteins. Although its predicted MW is 73 kDa, it runs anomalously at 100-110 kDa in SDS-PAGE. It is expressed in megakaryocytes, T cells and fibroblasts. APBB1IP acts as a coordinator for Rap1 GTPase signaling. Rap1 both antagonizes Ras signaling, and is associated with beta 1 and beta 2 integrin-induced cell adhesion and cytoskeleton rearrangement. Within this context, APBB1IP operates as a cytosolic adaptor protein that induces integrin activation by recruiting talin and RAP1 to the cell membrane, and by mediating Profilin and VASP protein polymerizatiuon of actin. Human APBB1IP is 666 amino acids (aa) in length. It contains a poly-Pro sequence (aa 129-148), a Ras-association domain (aa 176-263), a PH domain (aa 310-419), and a large poly-Pro region (aa 503-640). There are multiple potential isoform variants. One contains a deletion of aa 152-177 coupled to a 27 aa substitution for aa 262-666, a second possesses a 21 aa substitution for aa 152‑666, and a third shows a deletion of aa 386-390 coupled to a five aa substitution for aa 417-666. Over aa 2-89, human APBB1IP shares 97% aa sequence identity with mouse APBB1IP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RIAM/APBB1IP Antibody (AF7279)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol APBB1IP
Uniprot