Rhox8 Antibody - BSA Free

Immunohistochemistry: Rhox8 Antibody [NBP2-23671] - analysis of Rhox8 in Bouins-fixed Mouse testis using anti-Rhox8 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Rhox8 Antibody [NBP2-23671] - Immunolocalization of Rhox-8 protein in mice. Serial ovarian sections from eCG primed mice, after 8 h hCG-treated mice were incubated with either preimmune serum (1:250 dilution, A) or Rhox-8 antibody (1:2500 dilution, B).

Immunohistochemistry-Paraffin: Rhox8 Antibody [NBP2-23671] - Visualization by diaminobenzidine staining reveals that most of the Rhox-8 protein is localized to granulosa cells of large antral follicles (granulosa cells are shown at higher magnification in C-;F). Weak Rhox-8 protein staining can be observed in some granulosa cells of preantral follicles (C, arrowheads) and is absent in cumulus granulosa cells (C and E, arrows) surrounding the oocyte (O). D) Nuclei of thecal cells (arrowheads) are also positive for Rhox-8. F) Rhox-8 expression remains in the corpus luteum (CL) of postovulatory follicles.

Product Discontinued

Rhox8 Antibody - BSA Free Summary

• Immunogen: Within the range of amino acids 30-60 of mouse Rhox-8 protein were used as the immunogen for the antibody.
• Predicted Species: Human (100%), Rat (100%), Canine (94%), Equine (100%), Monkey (100%), Bovine (94%), Chimpanzee (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry reported by customer review
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA 1:100-1:2000
  • Western Blot reported in scientific literature (PMID 25972016)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (89%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole sera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Alternate Names for Rhox8 Antibody - BSA Free

• reproductive homeobox 8
• Rhox8 reproductive homeobox 8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rhox8 Antibody (NBP2-23671)(2)

Publications using NBP2-23671

• Welborn JP, Davis MG, Ebers SD et al. Rhox8 Ablation in the Sertoli Cells Using a Tissue-Specific RNAi Approach Results in Impaired Male Fertility in Mice. Biol. Reprod. 2015-05-13 [PMID: 25972016] (WB, IHC-P, Mouse)
• Brown RM, Davis MG, Hayashi K, MacLean JA. Regulated expression of Rhox8 in the mouse ovary: evidence for the role of progesterone and RHOX5 in granulosa cells. Biol Reprod. 2013-05-23 [PMID: 23536368] (IHC-P)
  
  Details:
  Imgenex antibodies cited used for IHC-p in granulosa cells: RHOX8 (IMX-5966). See Figs 2 and 6.

Review for Rhox8 Antibody (NBP2-23671) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: James MacLean ICC Mouse 06/29/2015

Summary

• Application: Immunocytochemistry
• Species: Mouse
• Lot: 09092223B