RHOT2 Antibody

Images

 
Western Blot: RHOT2 Antibody [NBP1-88982] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: RHOT2 Antibody [NBP1-88982] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human small intestine shows cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Simple Western: RHOT2 Antibody [NBP1-88982] - Simple Western lane view shows a specific band for RHOT2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: RHOT2 Antibody [NBP1-88982] - Electropherogram image(s) of corresponding Simple Western lane view. RHOT2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RHOT2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:RSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGACGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILC
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
RHOT2 Protein (NBP1-88982PEP)
Publications
Read Publication using
NBP1-88982 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24671417).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RHOT2 Antibody

  • ARHT2
  • C16orf39
  • chromosome 16 open reading frame 39
  • EC 3.6.5
  • EC 3.6.5.-
  • hMiro-2
  • MIRO-2mitochondrial Rho 2
  • mitochondrial Rho GTPase 2
  • Ras homolog gene family member T2
  • ras homolog gene family, member T2
  • RASL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RHOT2 Antibody (NBP1-88982)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RHOT2