Rhot1 Antibody

Images

 
Western Blot: Rhot1 Antibody [NBP1-59021] - Fetal Lung lysates, Antibody Dilution: 1.0 ug/ml.
Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-59021] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.
Western Blot: Rhot1 Antibody [NBP1-59021] - HepG2 cell lysate, concentration 1.0ug/ml.
Western Blot: Rhot1 Antibody [NBP1-59021] - RHOT1 Antibody Titration: 2 ug/ml Positive Control: mouse brain/ human neuroblastoma cell line
Western Blot: Rhot1 Antibody [NBP1-59021] - Antibody Titration: 1 ug/ml Human A549.
Western Blot: Rhot1 Antibody [NBP1-59021] - Fetal Lung lysates, Antibody Dilution: 1.0 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rhot1 Antibody Summary

Immunogen
Synthetic peptides corresponding to RHOT1(ras homolog gene family, member T1) The peptide sequence was selected from the N terminal of RHOT1 (NP_060777). Peptide sequence MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPER.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RHOT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 4-8 ug/ml
Application Notes
This is a rabbit polyclonal antibody against RHOT1 and was validated on Western Blot and immunohistochemistry-paraffin
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Rhot1 Lysate (NBP2-65343)
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-59021 in the following applications:

Publications
Read Publications using
NBP1-59021 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Rhot1 Antibody

  • ARHT1
  • EC 3.6.5
  • EC 3.6.5.-
  • FLJ11040
  • FLJ12633
  • hMiro-1
  • Miro1
  • MIRO-1mitochondrial Rho GTPase 1
  • mitochondrial Rho 1
  • rac-GTP binding protein-like protein
  • Rac-GTP-binding protein-like protein
  • Ras homolog gene family member T1
  • ras homolog gene family, member T1
  • Rhot1

Background

Mitochondrial GTPase involved in mitochondrial trafficking. RHOT1 is probably involved in control of anterograde transport of mitochondria and their subcellular distribution.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhot1 Antibody (NBP1-59021)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
All Applications
Filter By Species
Human
(1)
Rat
(1)
All Species

Reviews for Rhot1 Antibody (NBP1-59021) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-59021:
Filter by Applications
WB
(3)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Rhot1 NBP1-59021
Enlarge
reviewed by:
Anonymous
WB Human 03/07/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman whole cell lysates
SpeciesHuman
LotQC41365

Blocking

Blocking Details1 hr using 5% milk at room temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#1706515
Secondary Concentration1:10,000

Details

Detection NotesECL based detection using Chemidoc MP, 2 minutes.
Immunohistochemistry-Paraffin Rhot1 NBP1-59021
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 06/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse lung sections paraffin embedded
SpeciesMouse
LotQC41365
Western Blot Rhot1 NBP1-59021
Enlarge
reviewed by:
Anonymous
WB Mouse 06/02/2015
View

Summary

ApplicationWestern Blot
Sample TestedMouse lung homogenates
SpeciesMouse
LotQC41365

Blocking

Blocking Details5% Milk at Room Temperature for 1 hr

Primary Anitbody

Dilution Ratio1:1000 dilution at 4 degree for 16 hrs

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP secondary Ab.
Secondary Manufacturer Cat#170-6515
Secondary Concentration1:10,000

Details

Detection NotesBio-Rad Chemidoc MP, 10-60 sec exposure
Western Blot Rhot1 NBP1-59021
Enlarge
reviewed by:
Anonymous
WB Human 05/29/2015
View

Summary

ApplicationWestern Blot
Sample TestedLung homogenates
SpeciesHuman
LotQC41365

Blocking

Blocking Details5% Milk at Room Temperature for 1 hr

Primary Anitbody

Dilution Ratio1:1000 dilution at 4 degree for 16 hrs

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP secondary Ab.
Secondary Manufacturer Cat#170-6515
Secondary Concentration1:10,000

Details

Detection NotesBio-Rad Chemidoc MP, 10-60 sec exposure

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rhot1 Antibody (NBP1-59021) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Rhot1 Antibody (NBP1-59021)

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Research Areas for Rhot1 Antibody (NBP1-59021)

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Blogs on Rhot1

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Calculators

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Recent Reviews

4
5
0
4
4
3
0
2
0
1
0

Anonymous
03/07/2016
Application: WB
Species: Human

Anonymous
06/02/2015
Application: IHC-P
Species: Mouse

Anonymous
06/02/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol RHOT1
Entrez
Uniprot