RhoH Antibody (OTI9F4)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: OTI9F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RhoH Antibody (OTI9F4) Summary

• Description: Novus Biologicals Mouse RhoH Antibody (OTI9F4) (NBP3-44854) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 97-190 of human RHOH(NP_004301) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RHOH
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 degrees C. Avoid freeze/thaw cycles.
• Buffer: Lyophilized powder ( 1X PBS, pH 7.3,)
• Preservative: 8% Trehalose
• Purity: >95%
• Reconstitution Instructions: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RhoH Antibody (OTI9F4)

• ras homolog gene family, member H
• translocation three four

Background

The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOH