RhoGDI Antibody

Western Blot: RhoGDI Antibody [NBP1-52064] - (1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RhoGDI Antibody Summary

• Immunogen: Peptide with sequence C-QLAQIAAENE corresponding to internal region according to NP_004300.1.
• Epitope: C-QLAQIAAENE
• Predicted Species: Human (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGDIA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in mouse and rat lung lysates (calculated MW of 23.4 kDa band according to mouse NP_598557.3). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RhoGDI Antibody

• GDIA1
• MGC117248
• Rho GDI 1
• Rho GDP dissociation inhibitor (GDI) alpha
• rho GDP-dissociation inhibitor 1
• Rho-GDI alpha
• RHOGDI
• RHOGDI-1

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RhoGDI Antibody (NBP1-52064)(1)

Publication using NBP1-52064

• Ronneburg H, Span PN, Kantelhardt E et al. Rho GDP dissociation inhibitor alpha expression correlates with the outcome of CMF treatment in invasive ductal breast cancer. Int J Oncol 2010-02-01 [PMID: 20043072]

Bioinformatics

• Gene Symbol: ARHGDIA
• Entrez:
  • 396(Human)
  • 192662(Mouse)
• Uniprot:
  • NP_004300.1(Human)