RhoGDI Antibody

Images

 
Western Blot: RhoGDI Antibody [NBP1-52064] - (1ug/ml) staining of Mouse Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

RhoGDI Antibody Summary

Immunogen
Peptide with sequence C-QLAQIAAENE corresponding to internal region according to NP_004300.1.
Epitope
C-QLAQIAAENE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ARHGDIA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 28 kDa band observed in mouse and rat lung lysates (calculated MW of 23.4 kDa band according to mouse NP_598557.3). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
Publications
Read Publication using NBP1-52064.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RhoGDI Antibody

  • GDIA1
  • MGC117248
  • Rho GDI 1
  • Rho GDP dissociation inhibitor (GDI) alpha
  • rho GDP-dissociation inhibitor 1
  • Rho-GDI alpha
  • RHOGDI
  • RHOGDI-1

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoGDI Antibody (NBP1-52064)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGDIA
Entrez
Uniprot