RhoG Antibody (PSH02-70)

Western Blot: RhoG Antibody (PSH02-70) [NBP3-32902] - Western blot analysis of RhoG on different lysates with Rabbit anti-RhoG antibody (NBP3-32902) at 1/2,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: K-562 cell lysate Lane 4: Hela cell lysate Lane 5: MCF7 cell lysate Lane 6: PANC-1 cell lysate Lane 7: COS-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32902) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: RhoG Antibody (PSH02-70) [NBP3-32902] - Flow cytometric analysis of HeLa cells labeling RhoG. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32902, 1/1000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Pm
• Applications: WB, Flow
• Clone: PSH02-70
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RhoG Antibody (PSH02-70) Summary

• Description: Novus Biologicals Rabbit RhoG Antibody (PSH02-70) (NBP3-32902) is a recombinant monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human RhoG aa 1-50 / 191. (Uniprot: P84095)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RHOG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Western Blot 1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RhoG Antibody (PSH02-70)

• ARHGMGC125835
• MGC125836
• ras homolog gene family, member G (rho G)
• RhoG
• rho-related GTP-binding protein RhoG

Background

RhoG, also known as Rho-related GTP-binding protein RhoG, is a 21kDa 191 amino acid protein. RhoG plays a significant part in cell migration. RhoG has been researched in relation to Tangier disease, intrahepatic cholangiocarcinoma, shigellosis, cholangiocarcinoma, cholesterol and thyroiditis. RhoG has been linked to the axon guidance, cell adhesion, phagocytosis, cell motility and cell growth pathways where it interacts with VAV1, VAV2, ELMO1, MAP3K11 and ARHGDIG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RHOG