ELMO2 Antibody

Western Blot: ELMO2 Antibody [NB100-879] - Analysis of ELMO in Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: ELMO2 Antibody [NB100-879] - Staining of Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ELMO2 Antibody Summary

• Description: Novus Biologicals Goat ELMO2 Antibody (NB100-879) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-ELMO2 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PKEPSSYDFVYHYG corresponding to C-Terminus according to NP_877496.1, NP_573403.1.
• Epitope: C-PKEPSSYDFVYHYG
• Specificity: NP_877496.1 and NP_573403.1 are varients that encode the same protein.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ELMO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 80 kDa band observed in human brain lysate. IHC-P: Human prostate shows pixulated staining in the cytoplasm of stroma cells.
• Control:

  ELMO1 Overexpression Lysate

• Control Peptide:

  ELMO2 Peptide (NB100-879PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ELMO2 Antibody

• ced-12 homolog 2
• CED12A
• ELMO-2
• engulfment and cell motility 2 (ced-12 homolog, C. elegans)
• engulfment and cell motility 2
• engulfment and cell motility protein 2
• FLJ11656
• hCed-12A
• KIAA1834CED12CED-12
• PH domain protein CED12A
• Protein ced-12 homolog A

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ELMO2 Antibody (NB100-879)(5)

Publications using NB100-879

• Hernandez-Vasquez MN, Adame-Garcia SR, Hamoud N et al. Cell adhesion controlled by adhesion G-protein coupled receptor GPR124/ADGRA2 is mediated by a protein complex comprising Intersectins and Elmo/Dock. J. Biol. Chem. 2017-06-09 [PMID: 28600358]
• Abu-Thuraia A, Gauthier R, Chidiac R et al. Axl phosphorylates Elmo scaffold proteins to promote Rac activation and cell invasion. Mol. Cell. Biol. 2014-10-20 [PMID: 25332238] (WB, Human)
• Margaron Y, Fradet N, Cote JF. ELMO recruits Actin Crosslinking Family 7 (ACF7) at the cell membrane for microtubule capture and stabilization of cellular protrusions J Biol Chem 2012-11-26 [PMID: 23184944] (ICC/IF, Human)
• Ho E, Dagnino L. Epidermal growth factor induction of front-rear polarity and migration in keratinocytes is mediated by integrin-linked kinase and ELMO2. Mol Biol Cell. 2012-02-01 [PMID: 22160594]
• Gumienny TL, Brugnera E, Tosello-Trampont AC et al. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 2001-10-05 [PMID: 11595183]

Bioinformatics

• Gene Symbol: ELMO2
• Entrez:
  • 63916(Human)
  • 140579(Mouse)
• Uniprot:
  • NP_877496.1(Human)