ELMO2 Antibody

Images

 
Western Blot: ELMO2 Antibody [NB100-879] - Analysis of ELMO in Human Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ELMO2 Antibody [NB100-879] - Staining of Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ELMO2 Antibody Summary

Immunogen
Peptide with sequence C-PKEPSSYDFVYHYG corresponding to C-Terminus according to NP_877496.1, NP_573403.1.
Epitope
C-PKEPSSYDFVYHYG
Specificity
NP_877496.1 and NP_573403.1 are varients that encode the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ELMO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 80 kDa band observed in human brain lysate. IHC-P: Human prostate shows pixulated staining in the cytoplasm of stroma cells.
Positive Control
ELMO1 Lysate (NBL1-10239)
Publications
Read Publications using
NB100-879 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ELMO2 Antibody

  • ced-12 homolog 2
  • CED12A
  • ELMO-2
  • engulfment and cell motility 2 (ced-12 homolog, C. elegans)
  • engulfment and cell motility 2
  • engulfment and cell motility protein 2
  • FLJ11656
  • hCed-12A
  • KIAA1834CED12CED-12
  • PH domain protein CED12A
  • Protein ced-12 homolog A

Background

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELMO2 Antibody (NB100-879)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ELMO2 Antibody (NB100-879) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELMO2
Entrez
Uniprot