RFX6 Overexpression Lysate (Native)

Images

 
Western Blot: RFX6 Overexpression Lysate (Adult Normal) [NBP2-05116] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RFX6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

RFX6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_775831. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens RFX6, mRNA.
Gene
RFX6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
102.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for RFX6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for RFX6 Overexpression Lysate (Native)

  • dJ955L16.1
  • DNA-binding protein RFX6
  • MGC33442
  • MTFS
  • Regulatory factor X 6
  • Regulatory factor X domain-containing protein 1
  • regulatory factor X, 6
  • RFX6
  • RFXDC1
  • RFXDC1regulatory factor X domain containing 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3444
Species: Hu
Applications: WB
AF1837
Species: Hu
Applications: WB, Simple Western, ICC
AF6014
Species: Hu, Mu, Rt
Applications: WB
AF2419
Species: Hu
Applications: WB, Simple Western, IHC, ICC
AF7068
Species: Hu
Applications: WB, IHC, ICC
MAB4605
Species: Hu
Applications: WB, Simple Western, ICC, ICFlow
AF2400
Species: Hu
Applications: WB, ChIP, ICC
NBP2-45269
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB300-750
Species: Hu, Mu, Rt, Mk, Pm, Sh
Applications: WB, ICC/IF, IP
NB100-1793
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
NBP1-83449
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-82492
Species: Hu
Applications: WB, IHC, IHC-P
NB120-22104
Species: Hu, Mu, Rt
Applications: WB
NBP1-82135
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
NBP1-33596
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF2746
Species: Hu, Mu
Applications: WB, ICC
NBP1-89680
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-31348
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-05116
Species: Hu
Applications: WB

Publications for RFX6 Lysate (NBP2-05116) (0)

There are no publications for RFX6 Lysate (NBP2-05116).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for RFX6 Lysate (NBP2-05116) (0)

There are no reviews for RFX6 Lysate (NBP2-05116). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RFX6 Lysate (NBP2-05116) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional RFX6 Products

Bioinformatics Tool for RFX6 Lysate (NBP2-05116)

Discover related pathways, diseases and genes to RFX6 Lysate (NBP2-05116). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for RFX6 Lysate (NBP2-05116)

Discover more about diseases related to RFX6 Lysate (NBP2-05116).
 

Pathways for RFX6 Lysate (NBP2-05116)

View related products by pathway.

Blogs on RFX6

There are no specific blogs for RFX6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our RFX6 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol RFX6
Entrez