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RFC4 Antibody

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Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody NB100-233 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells ...read more
Western Blot: RFC4 Antibody [NB100-233] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human BRD4 (NBP1-18874). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human ATAD5 (NB100-57495). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anit-human RFC4 (NB100-233) and rabbit anti-human BRCA1 (NB100-61617). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human CTF18 (NBP1-03346). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human HDAC1 (NB100-2241). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RFC3 (NB100-435). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RFC4 (NB100-403). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human phospho-RPA2 [S33] (NB100-544). Image merged from DAPI (2ms) ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human phospho-RPA2 [S4/S8] (NBP1-23017). Image merged from DAPI ...read more
Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and Texas ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PLA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

      

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RFC4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RFC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • Proximity Ligation Assay 1:1000-1:20000
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RFC4 Lysate (NBL1-15294)
Publications
Read Publication using NB100-233.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RFC4 Antibody

  • A1 37 kDa subunit
  • A1
  • Activator 1 37 kDa subunit
  • Activator 1 subunit 4
  • MGC27291
  • replication factor C (activator 1) 4, 37kDa
  • Replication factor C 37 kDa subunit
  • replication factor C subunit 4
  • RFC 37 kDa subunit
  • RF-C 37 kDa subunit
  • RFC37replication factor C (activator 1) 4 (37kD)

Background

The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5. RFC4 is also known as replication factor C 37 kDa subunit, RF-C 37 kDa subunit, a1 37 kDa subunit, A1, RFC37, and MGC27291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RFC4 Antibody (NB100-233)(1)

We have publications tested in 1 confirmed species: Human.


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Positive Control Lysate(s)

RFC4 Overexpression Lysate (Native)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RFC4
Entrez
Uniprot