Biological Strategies: Western Blot: RFC4 Antibody [NB100-233] - Detection of Human and Mouse RFC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells ...read more
Western Blot: RFC4 Antibody [NB100-233] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.
40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The Replication factor C (RFC) heteropentamer includes RFC1, RFC2, RFC3, RFC4, and RFC5. The RFC heteropentamer is part of the multiprotein DNA replicase that functions to replicate DNA prior to cell division and repair DNA damage. RFC is the clamp loader ATPase associated with the DNA replicase. As the clamp loader, RFC uses ATP to recruit, open, and close the PCNA ring to link it to DNA for replication and repair. RFC4 is the 37 kDa subunit of RFC that is part of the core complex formed with RFC2 and RFC5. RFC4 is also known as replication factor C 37 kDa subunit, RF-C 37 kDa subunit, a1 37 kDa subunit, A1, RFC37, and MGC27291.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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