RFC4 Antibody (1C12) - Azide and BSA Free

Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 expression in transfected 293T cell line by RFC4 monoclonal antibody (M01), clone 1C12.Lane 1: RFC4 transfected lysate(39.7 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of monoclonal antibody to RFC4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - Analysis of RFC4 over-expressed 293 cell line, cotransfected with RFC4 Validated Chimera RNAi ( Cat # H00005984-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFC4 monoclonal antibody (M01), clone 1C12 (Cat # H00005984-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: RFC4 Antibody (1C12) [H00005984-M01] - RFC4 monoclonal antibody (M01), clone 1C12 Analysis of RFC4 expression in K-562.

Sandwich ELISA: RFC4 Antibody (1C12) [H00005984-M01] - Detection limit for recombinant GST tagged RFC4 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, Mycoplasma
• Clone: 1C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RFC4 Antibody (1C12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RFC4 (AAH17452, 254 a.a. ~ 363 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC
• Specificity: RFC4 - replication factor C (activator 1) 4, 37kDa
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RFC4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RFC4 Antibody (1C12) - Azide and BSA Free

• A1 37 kDa subunit
• A1
• Activator 1 37 kDa subunit
• Activator 1 subunit 4
• MGC27291
• replication factor C (activator 1) 4, 37kDa
• Replication factor C 37 kDa subunit
• replication factor C subunit 4
• RFC 37 kDa subunit
• RF-C 37 kDa subunit
• RFC37replication factor C (activator 1) 4 (37kD)

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFC4
• Entrez:
  • 5984(Human)
• OMIM:
  • 102577(Human)
• Uniprot:
  • AAH17452(Human)