RFC2 Antibody

Images

 
Western Blot: RFC2 Antibody [NBP1-32253] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RFC2 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: RFC2 Antibody [NBP1-32253] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Western Blot: RFC2 Antibody [NBP1-32253] - A: A431 B: H1299 C: Hela 10% SDS PAGE; antibody diluted at 1:1000

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RFC2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RFC2. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (96%), Rat (96%), Chicken (93%), Xenopus (91%), Rhesus Macaque (99%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RFC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for RFC2 Antibody

  • A1 40 kDa subunit
  • A1
  • Activator 1 40 kDa subunit
  • Activator 1 subunit 2
  • MGC3665
  • replication factor C (activator 1) 2, 40kDa
  • replication factor C 2
  • Replication factor C 40 kDa subunit
  • replication factor C subunit 2
  • RF-C 40 kDa subunit
  • RFC40replication factor C (activator 1) 2 (40kD)

Background

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RFC2