Ret Antibody [Unconjugated]

Ret was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human Ret Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1485) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motor neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ret Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ret
  Leu29-Arg635
  Accession # P07949
• Specificity: Detects human Ret in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Ret is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RET
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ret Antibody [Unconjugated]

• CDHF12MTC1
• CDHR16
• EC 2.7.10
• Hirschsprung disease 1
• Hirschsprung disease)
• MEN2A
• MEN2B
• multiple endocrine neoplasia and medullary thyroid carcinoma 1
• ret proto-oncogene
• RET transforming sequence
• Ret

Background

The GDNF family of neurotrophic factors forms a subfamily within the TGF-beta superfamily. These proteins are potent survival factors for various central and peripheral neurons during development and the adult animal. The GDNF family members (GDNF, neurturin, artemin and persephin) signal through multicomponent receptors that consist of the Ret receptor tyrosine kinase and one of four glycosyl-phosphatidylinositol (GPI)-linked ligand-binding subunits (GFR alpha ‑1‑4). GFR alpha -1, -2, -3, and -4 are the preferred ligand-binding subunits for GDNF, neurturin, artemin and persephin, respectively. The Ret tyrosine-kinase receptor is encoded by the c-ret proto-oncogene. Mutations of the ret gene have been associated with various human diseases affecting tissues derived from the neural crest, including Hirschsprung’s disease, multiple endocrine neoplasia MEN2A and MEN2B, and familial medullary thyroid carcinoma. Human and mouse Ret share 83% amino acid sequence homology (77% homology in the extracellular domain and 93% homology in the cytoplasmic domain). Although Ret does not bind GDNF ligands directly, the extracellular domain of Ret binds the GDNF-GFR-alpha complex with high affinity and is a potent GDNF antagonist in the presence of soluble GFR-alpha (1-4).

1. Trupp, M. et al. (1998) Mol. Cell. Neurosci. 11:47.
2. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
3. Carlomagno, F. et al. (1998) Endocrinology 139:3613.
4. Baloh, R. et al. (1998) Neuron 21:1291.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ret Antibody (AF1485)(11)

Publications using AF1485

• Shi, M;Crouse, B;Sundaram, N;Shakked, NP;Ester, L;Zhang, W;Janakiram, V;Kopan, R;Helmrath, MA;Bonventre, JV;McCracken, KW; Integrating collecting systems in kidney organoids through fusion of distal nephron to ureteric bud bioRxiv : the preprint server for biology 2024-09-20 [PMID: 39345524] (Immunocytochemistry, Human)
• Carpenter, J;Yarlagadda, S;VandenHeuvel, KA;Ding, L;Schuh, MP; Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants Kidney international reports 2024-02-01 [PMID: 38344733] (Immunohistochemistry, Human)
• TC Alich, P Röderer, B Szalontai, K Golcuk, S Tariq, M Peitz, O Brüstle, I Mody Bringing to light the physiological and pathological firing patterns of human induced pluripotent stem cell-derived neurons using optical recordings Frontiers in Cellular Neuroscience, 2023-01-17;16(0):1039957. 2023-01-17 [PMID: 36733665] (ICC, Human)
• R�derer P, Belu A, Heidrich L et al. Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell-derived sensory neurons Pain 8/1/2023 [PMID: 36727909]
• R�derer P, Belu A, Heidrich L et al. Emergence of nociceptive functionality and opioid signaling in human induced pluripotent stem cell-derived sensory neurons Pain 2023-08-01 [PMID: 36727909]
• Chu Y, Kordower J GDNF signaling in subjects with minimal motor deficits and Parkinson's disease. Neurobiol Dis, 2021-03-05;153(0):105298. 2021-03-05 [PMID: 33684514] (IHC, Cynomolgus Monkey)
• SI Mae, M Ryosaka, S Sakamoto, K Matsuse, A Nozaki, M Igami, R Kabai, A Watanabe, K Osafune Expansion of Human iPSC-Derived Ureteric Bud Organoids with Repeated Branching Potential Cell Rep, 2020-07-28;32(4):107963. 2020-07-28 [PMID: 32726627] (ICC, Human)
• AI Yu, L Zhao, KA Eaton, S Ho, J Chen, S Poe, J Becker, A Gonzalez, D McKinstry, M Hasso, J Mendoza-Ca, J Whitfield, C Koumpouras, PD Schloss, EC Martens, GY Chen Gut Microbiota Modulate CD8�T Cell Responses to Influence Colitis-Associated Tumorigenesis Cell Rep, 2020-04-07;31(1):107471. 2020-04-07 [PMID: 32268087] (ICC, Human)
• Chu Y, Bartus R, Manfredsson F, Olanow C, Kordower J Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson's disease. Brain, 2020-03-01;143(3):960-975. 2020-03-01 [PMID: 32203581] (IHC, Mouse)
• Menon R, Otto E, Kokoruda A, Zhou J, Zhang Z, Yoon E, Chen Y, Troyanskaya O, Spence J, Kretzler M, Cebrian C Single-cell analysis of progenitor cell dynamics and lineage specification in the human fetal kidney. Development, 2018-08-30;145(16):. 2018-08-30 [PMID: 30166318] (IHC, Human)
• Narita N, Tanemura A, Murali R, Scolyer RA, Huang S, Arigami T, Yanagita S, Chong KK, Thompson JF, Morton DL, Hoon DS Functional RET G691S polymorphism in cutaneous malignant melanoma. Oncogene, 2009-06-29;28(34):3058-68. 2009-06-29 [PMID: 19561646] (IHC-P, Neutralization, Human)

Reviews for Ret Antibody (AF1485) (5)

Average Rating: 5

(Based on 5 reviews)

We have 5 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IF Human 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Ret in neuroblastoma cells and of recombinant human Ret expressed in 293 cells and MG87 cells
• Species: Human

reviewed by: Verified Customer IP Human 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Endogenous Ret in neuroblastoma cells and of recombinant human Ret expressed in 293 cells and MG87 cells
• Species: Human

reviewed by: Verified Customer IF Mouse 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Endogenous Ret in mouse sections
• Species: Mouse

reviewed by: Verified Customer WB Mouse 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: mouse tissue homogenates
• Species: Mouse

reviewed by: Verified Customer WB Human 12/18/2014

Summary

• Application: Western Blot
• Sample Tested: 293 cells and MG87 cells transfected with human Ret
• Species: Human

Bioinformatics

• Gene Symbol: RET
• Uniprot:
  • P07949()