Human Resistin ELISA Kit (Colorimetric) Summary
| Description |
This kit is an enzyme-linked immunosorbent assay for quantitative detection. |
| Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Resistin positive serum. There was no crossreactivity detected. |
| Standard Curve Range |
31 - 2,000 pg/mL |
| Sensitivity |
3.1 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 8.1% |
| Intra-Assay |
CV% < 5.1% |
| Sample Volume |
50 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
RETN |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Resistin
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-human Resistin polyclonal antibody
- Human Resistin Standard lyophilized, 4000 pg/ml upon reconstitution
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Human Resistin ELISA Kit (Colorimetric)
Background
Resistin is a hormone secreted by adipose tissue. The length of the resistin pre-peptide in human is 108 aminoacids; the molecular weight is ~12.5 kDa. Resistin was originally found to be produced and released from adipose tissue to serve endocrine functions likely involved in insulin resistance. This idea primarily stems from studies demonstrating that serum resistin levels increase with obesity in several model systems. Since these observations, further research has linked resistin to other physiological systems such as inflammation and energy homeostasis. Current research is proposing to link resistin to inflammation and energy homeostasis, including its alleged role in insulin resistance in obese subjects.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Resistin ELISA Kit (NBP1-83744). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.