Human Resistin ELISA Kit (Colorimetric)

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ELISA: Resistin ELISA Kit [NBP1-83744] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
31 - 2,000 pg/mL
Sensitivity
3.1 pg/mL

Order Details

Human Resistin ELISA Kit (Colorimetric) Summary

Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human Resistin positive serum. There was no crossreactivity detected.
Standard Curve Range
31 - 2,000 pg/mL
Sensitivity
3.1 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.1%
Intra-Assay
5.1%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
RETN

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to human Resistin
  2. Adhesive Films
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Human Resistin Standard lyophilized, 4000 pg/ml upon reconstitution
  5. Biotin-Conjugate anti-human Resistin polyclonal antibody
  6. Stop Solution (1M Phosphoric acid)
  7. Substrate Solution (tetramethyl-benzidine)
  8. Streptavidin-HRP
  9. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Alternate Names for Human Resistin ELISA Kit (Colorimetric)

  • Adipose tissue-specific secretory factor
  • ADSF
  • ADSFMGC126609
  • C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1
  • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
  • Cysteine-rich secreted protein A12-alpha-like 2
  • Cysteine-rich secreted protein FIZZ3
  • FIZZ3
  • FIZZ3MGC126603
  • found in inflammatory zone 3
  • HXCP1
  • Resistin
  • RETN
  • RETN1
  • RSTNXCP1

Background

Resistin is a hormone secreted by adipose tissue. The length of the resistin pre-peptide in human is 108 aminoacids; the molecular weight is ~12.5 kDa. Resistin was originally found to be produced and released from adipose tissue to serve endocrine functions likely involved in insulin resistance. This idea primarily stems from studies demonstrating that serum resistin levels increase with obesity in several model systems. Since these observations, further research has linked resistin to other physiological systems such as inflammation and energy homeostasis. Current research is proposing to link resistin to inflammation and energy homeostasis, including its alleged role in insulin resistance in obese subjects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RETN
Entrez