Renin Antibody (434703)

Renin was detected in perfusion fixed frozen sections of mouse kidney using 25 µg/mL Rat Anti-Mouse Renin 1 Propeptide Monoclonal Antibody (Catalog # MAB42771) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Product Discontinued

Renin Antibody (434703) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Renin 1
  Leu22-Arg402
  Accession # P06281
• Specificity: Detects mouse Renin 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Renin, recombinant mouse (rm) Cathepsin D, or rmCathepsin E is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: REN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Renin Antibody (434703)

• angiotensin-forming enzyme
• Angiotensinogenase
• EC 3.4.23
• EC 3.4.23.15
• FLJ10761
• HNFJ2
• Prorenin
• REN
• renin precursor, renal
• Renin

Background

Mouse Renin is a secreted, 42‑47 kDa glycosylated member of the peptidase A1 family. It is an aspartyl protease that cleaves angiotensinogen to form angiotensin I. In mouse, there are two genes that code for Renin. One is in the submandibular gland and the other is in the kidney. The two mature Renin molecules share 95% amino acid (aa) sequence identity. Renal Renin (Renin‑1) is synthesized as a 381 aa proform (aa 22‑402). In the kidney, pro‑Renin is proteolytically cleaved after Thr71 to generate a mature enzyme. Mouse pro‑Renin shares 70% and 85% aa sequence identity with human and rat pro‑Renin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Renin Antibody (MAB42771)(1)

Publication using MAB42771

• Charlotte Starke, Hannah Betz, Linda Hickmann, Peter Lachmann, Björn Neubauer, Jeffrey B. Kopp et al. Renin Lineage Cells Repopulate the Glomerular Mesangium after Injury Journal of the American Society of Nephrology 2015-01-01 [PMID: 24904091] (Immunohistochemistry, Mouse)

Bioinformatics

• Gene Symbol: REN
• Uniprot:
  • P06281()