Western Blot: NFkB p65 Antibody [NBP1-96139] - WB analysis of NFkB p65 in: 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.
Immunocytochemistry/ Immunofluorescence: NFkB p65 Antibody [NBP1-96139] - NFKB p65 antibody was tested in COS-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NFkB p65 Antibody [NBP1-96139] - IHC analysis of NFkB p65 in mouse brain using DAB with hematoxylin counterstain.
A synthetic peptide made to an internal portion of the human NFkB p65 protein (between residues 300-400) [UniProt Q04206]
Note: Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.05% Sodium Azide
Immunogen affinity purified
This NFkB p65 antibody is useful for Western blot where a band is seen ~60 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP1-96139 in the following application:
NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
NFkB is a pleiotropic transcription factor which exists as homodimeric/heterodimeric complex generated by Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52, and years of research has established that p65-p50 complex is most abundant in various cell types. The dimers bind at kB sites in their target genes DNA and the individual dimers exhibits differential affinity/specificity for acting as transcriptional activators or repressors e.g. p65-p50 and p65-c-Rel dimers acts transcriptional activators. Inside cytoplasm, NFkB complexes are held in an inactive state complexed with NFkB inhibitor or IkB family members which gets phosphorylated through IKKs/IkB kinases in response to different activators, subsequently degraded thus liberating the active NFkB complex which translocates to the nucleus for modulating the target genes. NFkB it has been found as the endpoint of a series of signaling events which are initiated by a diverse array of stimuli associated with critical cellular processes such as inflammation, immunity, differentiation, cell growth, apoptosis and tumorigenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
NFkB3-p65: Say that three times fast! NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus... Read full blog post.