RelA/NFkB p65 Antibody

Images

 
Western Blot: NFkB p65 Antibody [NBP1-96139] - WB analysis of NFkB p65 in: 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.
Immunocytochemistry/ Immunofluorescence: NFkB p65 Antibody [NBP1-96139] - NFKB p65 antibody was tested in COS-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: NFkB p65 Antibody [NBP1-96139] - IHC analysis of NFkB p65 in mouse brain using DAB with hematoxylin counterstain.

Product Details

Summary
Product Discontinued
View other related RelA/NFkB p65 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-96139
    • Availability
      Product Discontinued

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RelA/NFkB p65 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human NFkB p65 protein (between residues 300-400) [UniProt Q04206]
Localization
Nucleus. Cytoplasm. Note: Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
This NFkB p65 antibody is useful for Western blot where a band is seen ~60 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-96139 in the following applications:

Publications
Read Publications using
NBP1-96139 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, mouse, and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.02 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RelA/NFkB p65 Antibody

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

NFkB is a pleiotropic transcription factor which exists as homodimeric/heterodimeric complex generated by Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52, and years of research has established that p65-p50 complex is most abundant in various cell types. The dimers bind at kB sites in their target genes DNA and the individual dimers exhibits differential affinity/specificity for acting as transcriptional activators or repressors e.g. p65-p50 and p65-c-Rel dimers acts transcriptional activators. Inside cytoplasm, NFkB complexes are held in an inactive state complexed with NFkB inhibitor or IkB family members which gets phosphorylated through IKKs/IkB kinases in response to different activators, subsequently degraded thus liberating the active NFkB complex which translocates to the nucleus for modulating the target genes. NFkB it has been found as the endpoint of a series of signaling events which are initiated by a diverse array of stimuli associated with critical cellular processes such as inflammation, immunity, differentiation, cell growth, apoptosis and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NBP1-96139)(3)

Review for RelA/NFkB p65 Antibody (NBP1-96139) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-96139:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedH1299 whole cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST, overnight @ 4C

Primary Anitbody

Dilution Ratio1:2000

Secondary Antibody

Secondary Descriptionanti-rabbit
Secondary Manufacturer Cat#Cell signaling
Secondary Concentration1:5000

Details

Detection NotesECL reagent, 2 mins

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RelA/NFkB p65 Antibody (NBP1-96139) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RelA/NFkB p65 Products

Bioinformatics Tool for RelA/NFkB p65 Antibody (NBP1-96139)

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Diseases for RelA/NFkB p65 Antibody (NBP1-96139)

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Pathways for RelA/NFkB p65 Antibody (NBP1-96139)

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PTMs for RelA/NFkB p65 Antibody (NBP1-96139)

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Research Areas for RelA/NFkB p65 Antibody (NBP1-96139)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RELA
Entrez
Uniprot