Reg3A Overexpression Lysate (Native)

Images

 
Western Blot: Reg3a Overexpression Lysate (Adult Normal) [NBL1-15274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Reg3a.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Reg3A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216966 and based on accession number NM_138937. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens regenerating islet-derived 3 alpha (REG3A), transcript variant 3, mRNA.
Gene
REG3A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Reg3A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Reg3A Overexpression Lysate (Native)

  • hepatocarcinoma-intestine-pancreas
  • HIPpancreatitis-associated protein
  • Human proislet peptide
  • Lithostathine 3
  • pancreatic beta cell growth factor
  • Pancreatitis-associated protein 1
  • PAP homologous protein
  • PAP1FLJ18565
  • PAP2
  • PAPPAP-H
  • PBCGF
  • proliferation-inducing protein 34
  • proliferation-inducing protein 42
  • Reg III-alpha
  • REG3
  • Reg3A
  • REG-3-alpha
  • regenerating islet-derived 3 alpha
  • regenerating islet-derived protein 3-alpha
  • Regenerating islet-derived protein III-alpha
  • REG-III

Background

This gene encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. Multiple alternatively spliced transcript variants encoding the same protein have been described for this gene but the full length nature of some transcripts is not yet known. Transcript Variant: This variant (3) uses alternate splice site in exon 2 of the 5' UTR, as compared to variant type 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Reg3A Lysate (NBL1-15274) (0)

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Bioinformatics

Gene Symbol REG3A
Entrez