Reelin Antibody (RELN/4498) - Azide and BSA Free

Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) - Azide and BSA Free [NBP3-14203] - Formalin-fixed, paraffin-embedded human kidneystained with Reelin antibody (RELN/4498). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu, Mu, Ma
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: RELN/4498
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Reelin Antibody (RELN/4498) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13874).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant fragment (around aa 1796-2115) of human Reelin protein (Uniprot: P78509)
• Localization: Cytoplasm
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RELN
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Immunoprecipitation 1-2 ug per 100-500 ug protein
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order this antibody without BSA
  
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
  
  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
• Theoretical MW: 387.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rodent

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Reelin Antibody (RELN/4498) - Azide and BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RELN