Reelin Antibody (142) - BSA Free

Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin staining of Reelin-positive Cajal-Retzius neurons in a human fetal cerebral cortex at 21 weeks of gestation, using anti-Reelin MAB5366 (dilution 1:500). Photo courtesy of Dr. G. Meyer.

Immunohistochemistry: Reelin Antibody (142) [NB600-1080] - Mouse anti-Reelin visualization of a Cajal-Retzius neuron from a human newborn stained for reelin using MAB5366. This photo is courtesy of Dr. G. Meyer.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 142
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Reelin Antibody (142) - BSA Free Summary

• Description: Novus Biologicals Mouse Reelin Antibody (142) - BSA Free (NB600-1080) is a monoclonal antibody validated for use in IHC, WB and Simple Western. Anti-Reelin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant reelin amino acids 40-189.
• Localization: Secreted
• Specificity: Reelin. The epitope is between amino acids 164 and 189.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RELN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:500
  • Simple Western 1:50
  • Western Blot 1:250-1:500
• Application Notes: Western Blot: Reelin can appear as three bands ~400 - 450, 300, and 180 - 200 kDa's.

Reactivity Notes

React in most vertebrates. Rat reactivity reported in scientific literature (PMID: 22252605). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Phosphate buffer and 0.25M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Reelin Antibody (142) - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reelin Antibody (NB600-1080)(1)

Publication using NB600-1080

• Ogawa B, Wang L, Ohishi T, Taniai E, Akane H, Suzuki K, Mitsumori K, Shibutani M. Reversible aberration of neurogenesis targeting late-stage progenitor cells in the hippocampal dentate gyrus of rat offspring after maternal exposure to acrylamide. Arch Toxicol;86(5):779-90. 2012-05-01 [PMID: 22252605] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: RELN
• Entrez:
  • 5649(Human)
• Uniprot:
  • P78509()