Recombinant Zebrafish BMP-2a Protein

• Reactivity: Ze
• Applications: Bioactivity

Recombinant Zebrafish BMP-2a Protein Summary

• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 100-400 ng/mL.
• Source: E. coli-derived zebrafish BMP-2a protein
  Gln272-Arg386, with an N-terminal Met
• Accession #: AAI63048.1
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: bmp2a
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Zebrafish BMP-2a Protein

• BMP2a
• BMP-2a

Background

BMP-2 is one of at least 20 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily (1, 2). BMPs were originally indentified as protein regulators of cartilage and bone formation. However, they are also involved in embryogenesis and morphogenesis of various tissues and organs. BMPs regulate the growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse, and rat BMP-2 are 100% identical, while mature human BMP-2 and zebrafish BMP-2a are 85% identical. Zebrafish have another homolog of BMP-2a, BMP-2b, which is 88% identical to BMP-2a in the mature region and corresponds to the swirl mutant. The combined expression pattern of zBMP-2a/2b/4 coincides with areas where BMP-2/4 expression would be found in other vertebrates (3). Biologically active BMP-2a is a disulfide-linked homodimer of the carboxy-terminal 115 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2a have been shown to be mediated by the formation of hetero-ogliomeric complexes of type I and type II serine/threonine kinase receptors (4). In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type I’s can bind BMP-2 with high affinity in the absence of BMP receptor type II.

1. Ryoo, H.M. et al. (2006) Gene 366:51.
2. Chen, D. et al. (2004) Growth Factors 22:233.
3. Martinez-Barbera, J. et al. (1997) Gene 198:53.
4. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.

Publications for BMP-2a (111-BM)(1)

Publication using 111-BM

• Satoh A, Cummings GM, Bryant SV, Gardiner DM Neurotrophic regulation of fibroblast dedifferentiation during limb skeletal regeneration in the axolotl (Ambystoma mexicanum). Dev. Biol., 2009-11-24;337(2):444-57. 2009-11-24 [PMID: 19944088] (In Vivo, Amphibian)

Bioinformatics

• Gene Symbol: bmp2a
• Uniprot:
  • AAI63048.1()