Recombinant Rhesus Macaque IL-12 Protein


There are currently no images for IL-12 (3216-RL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity RMSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rhesus Macaque IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.07-0.3 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived rhesus macaque IL-12 protein
Rhesus Macaque IL-12 (p40)
(Ile23 - Ser328)
Accession # P48095
6-His tag
Rhesus Macaque IL-12 (p35)
(Arg57 - Ser253)
Accession # NP_001038199
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile23 (p40) & Arg57 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
35.6 kDa (p40), 23.4 kDa (p35).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
40-45 kDa and 36-37 kDa, reducing conditions
Read Publications using
3216-RL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rhesus Macaque IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12 (IL-12) is a 70 - 75 kDa heterodimeric glycoprotein that belongs to the IL-12 family of cytokines (1 - 3). Monkey IL-12 consists of two disulfide-linked subunits which are 35 kDa and 40 kDa in size. They show no meaningful amino acid (aa) similarity (4). The mature 35 kDa subunit (p35; also IL-12A) is an alpha -helix, 197 aa in length, that contains seven cysteines and two potential N-linked glycosylation sites (4). Mature Rhesus monkey p35 is 94%, 98%, 97%, 57%, 81%, and 82% aa identical to human, mangabey, baboon, mouse, equine and porcine mature p35, respectively. The mature 40 kDa subunit (p40; alsoIL-12B) is 306 aa in length and contains one intrachain disulfide bond plus three potential N-linked glycosylation sites. Mature Rhesus p40 is 96%, 97%, 99%, 67%, 85%, and 80% aa identical to human, mangabey, baboon, mouse, equine, and porcine p40, respectively. While p35 is reminescent of a hematopoietin ligand, p40 strongly resembles the N-terminus of a hematopoietin receptor, exhibiting a WSXWS motif, an immunoglobulin-like domain, and one fibronectin type III domain (5). This has led to the suggestion that IL-12 may be a cytokine-receptor analog to the IL-6/soluble IL-6R complex (6 - 8). Notably, while p40 may circulate as a monomer or homodimer, p35 is never found by itself. Cells known to produce IL-12 include macrophages and dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. Functionally, IL-12 has been shown to both enhance cytotoxic activity and induce interferon-gamma (IFN-gamma ) production in NK cells and T cells. In response to infection, IL-27 is released initially, promoting a Th0 to Th0/1 transition state. IL-12 follows next, generating Th1 effector cells. With IL-18, IL-12 creates Th1 memory cells out of effector cells (1 - 3, 6, 7).

  1. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  2. Langrish, C.L. et al. (2004) Immunol. Rev. 202:96.
  3. Kreymborg, K. et al. (2005) Expert Opin. Ther. Targets 9:1123.
  4. Villinger, F. et al. (1995) J. Immunol. 155:3946.
  5. GenBank Accession # P48095.
  6. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  7. Trinchieri, G. (2003) Nat. Rev. Immunol. 3:133.
  8. Gearing, D.P. and D. Cosman (1991) Cell 66:9.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu
Applications: WB, IHC, ICC
Species: Mu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO

Publications for IL-12 (3216-RL)(2)

We have publications tested in 1 confirmed species: Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 1 application: Bioassay.

Filter By Application
All Applications
Filter By Species
Primate - Macaca mulatta (Rhesus Macaque)
All Species

Reviews for IL-12 (3216-RL) (0)

There are no reviews for IL-12 (3216-RL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-12 (3216-RL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-12 Products

Diseases for IL-12 (3216-RL)

Discover more about diseases related to IL-12 (3216-RL).

Pathways for IL-12 (3216-RL)

View related products by pathway.

PTMs for IL-12 (3216-RL)

Learn more about PTMs related to IL-12 (3216-RL).

Blogs on IL-12.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes expres...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Rhesus Macaque IL-12 Protein and receive a gift card or discount.