Recombinant Rat ROBO1 Fc Chimera Protein, CF

• Reactivity: Rt
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Rat ROBO1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Biotinylated recombinant mouse Slit-3 immobilized on a streptavidin-coated plate at 1 µg/mL can bind Recombinant Rat ROBO1 Fc Chimera with an apparent K_d <10 nM.
• Source: Mouse myeloma cell line, NS0-derived rat ROBO1 protein
  

  Rat ROBO1 (Lys19-Ile560) Accession # O55005	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O55005
• N-terminal Sequence: Lys19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Robo1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 86.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat ROBO1 Fc Chimera Protein, CF

• DUTT1
• DUTT1Drosophila) homolog 1
• H-Robo-1
• MGC131599
• MGC133277
• ROBO1
• roundabout, axon guidance receptor, homolog 1 (Drosophila)

Background

ROBO1 is a 170-200 kDa member of the ROBO family of guidance molecules. It serves as a repulsing molecule that prevents axons from crossing the midline in the developing central nervous system (CNS) (1). Mature rat ROBO1 consists of an 872 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 733 aa cytoplasmic region (2). The ECD contains five tandem Ig-like domains followed by three tandem fibronectin type III domains. Usage of an alternative promoter for human ROBO1 generates a shorter isoform (ROBO1b or DUTT1) that has an 18 aa substitution for the N-terminal 57 residues (2, 3). Within the ECD, rat ROBO1 shares 98% and 97% aa identity with mouse and human ROBO1, respectively. A 120 kDa soluble fragment of the ECD can be shed by juxtamembrane proteolytic cleavage (4). ROBO1 is expressed on pioneer axons in the neural tube and proliferative zones of the cortical plate (5-8). It is restricted to axonal growth cones that do not cross the midline and to midline-crossing axons only after they cross (2). ROBO1b shows even wider expression in the developing mouse CNS and is the predominant isoform in spinal cord commissural axons (5). ROBO1 plays an important role in axon pathfinding and tract development (6, 9, 10) as well as in neuronal proliferation and dendrite branching (7, 8). ROBO1 binds to Slit1 and Slit2, leading to growth cone collapse and axonal branching (7, 11). It also regulates Netrin-1 and Semaphorin 3A and 3F responsiveness through its ability to form in cis complexes with Neuropilin-1, Neuropilin-2, Plexin A1, DCC, and FLRT3 (10, 12, 13). ROBO1 is additionally expressed in developing ductal epithelia where its binding to Slit2 restricts branching morphogenesis (14). ROBO1-Slit2 interactions can also suppress tumorigenesis (15), enhance VEGF-induced angiogenesis (16), and enhance CXCL12 induced T cell chemotaxis by associating with CXCR4 (17).

1. Dudanova, I and R. Klein (2013) Trends Neurosci. 36:295.
2. Kidd, T. et al. (1998) Cell 92:205.
3. Sundaresan, V. et al. (1998) Mol. Cell. Neurosci. 11:29.
4. Seki, M. et al. (2010) FEBS Lett. 584:2909.
5. Nural, H.F. et al. (2007) Gene Expr. Patterns 7:837.
6. Wang, G. et al. (2013) Exp. Cell Res. 319:1083.
7. Whitford, K.L. et al. (2002) Neuron 33:47.
8. Yeh, M.L. et al. (2014) J. Neurosci. 34:5717.
9. Andrews, W. et al. (2006) Development 133:2243.
10. Hernandez-Miranda, L.R. et al. (2011) J. Neurosci. 31:6174.
11. Liu, Z. et al. (2004) Mol. Cell. Neurosci. 26:232.
12. Stein, E. and M. Tessier-Lavigne (2001) Science 291:1928.
13. Leyva-Diaz, E. et al. (2014) Curr. Biol. 24:494.
14. Macias, H. et al. (2011) Dev. Cell 20:827.
15. Chang, P.H. et al. (2012) Cancer Res. 72:4652.
16. Rama, N. et al. (2015) Nat. Med. 21:483.
17. Prasad, A. et al. (2007) J. Leukoc. Biol. 82:465.

Publications for ROBO1 (1749-RB)(10)

Publications using 1749-RB

• Li, Q;Huang, L;Ding, Y;Sherchan, P;Peng, W;Zhang, JH; Recombinant Slit2 suppresses neuroinflammation and Cdc42-mediated brain infiltration of peripheral immune cells via Robo1-srGAP1 pathway in a rat model of germinal matrix hemorrhage Journal of neuroinflammation 2023-10-29 [PMID: 37899442] (In vivo assay, Rat)
• AN Gonçalves, J Correia-Pi, C Nogueira-S ROBO2 signaling in lung development regulates SOX2/SOX9 balance, branching morphogenesis and is dysregulated in nitrofen-induced congenital diaphragmatic hernia Respir Res, 2020-11-18;21(1):302. 2020-11-18 [PMID: 33208157] (Cell Culture, Rat)
• VK Bhosle, T Mukherjee, YW Huang, S Patel, BWF Pang, GY Liu, M Glogauer, JY Wu, DJ Philpott, S Grinstein, LA Robinson SLIT2/ROBO1-signaling inhibits macropinocytosis by opposing cortical cytoskeletal remodeling Nat Commun, 2020-08-17;11(1):4112. 2020-08-17 [PMID: 32807784] (Bioassay, Mouse)
• Yeh M, Gonda Y, Mommersteeg M, Barber M, Ypsilanti A, Hanashima C, Parnavelas J, Andrews W Robo1 modulates proliferation and neurogenesis in the developing neocortex. J Neurosci, 2014-04-16;34(16):5717-31. 2014-04-16 [PMID: 24741061] (Bioassay, Rat)
• Gohrig A, Detjen K, Hilfenhaus G, Korner J, Welzel M, Arsenic R, Schmuck R, Bahra M, Wu J, Wiedenmann B, Fischer C Axon guidance factor SLIT2 inhibits neural invasion and metastasis in pancreatic cancer. Cancer Res, 2014-01-21;74(5):1529-40. 2014-01-21 [PMID: 24448236] (Neutralization, Human, Mouse)
• Ricano-Cornejo I, Altick AL, Garcia-Pena CM, Nural HF, Echevarria D, Miquelajauregui A, Mastick GS, Varela-Echavarria A Slit-Robo signals regulate pioneer axon pathfinding of the tract of the postoptic commissure in the mammalian forebrain. J. Neurosci. Res., 2011-06-17;89(10):1531-41. 2011-06-17 [PMID: 21688288] (Bioassay, Mouse)
• Hernandez-Miranda LR, Cariboni A, Faux C, Ruhrberg C, Cho JH, Cloutier JF, Eickholt BJ, Parnavelas JG, Andrews WD Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain. J. Neurosci., 2011-04-20;31(16):6174-87. 2011-04-20 [PMID: 21508241] (Bioassay, Human)
• Brantley-Sieders DM, Dunaway CM, Rao M Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium. Cancer Res., 2010-12-08;71(3):976-87. 2010-12-08 [PMID: 21148069] (In Vivo, Mouse)
• Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2010-12-06;31(3):404-16. 2010-12-06 [PMID: 21135133] (Bioassay, Mouse)
• Geutskens SB, Hordijk PL, van Hennik PB The chemorepellent Slit3 promotes monocyte migration. J. Immunol., 2010-11-15;185(12):7691-8. 2010-11-15 [PMID: 21078908] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Robo1
• Uniprot:
  • O55005()