Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

• Reactivity: Rt
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rrNpn-1/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhVEGF₁₆₅ in the presence of 2 µg/mL of heparin with an apparent K_D <1 nM.
• Source: Mouse myeloma cell line, NS0-derived rat Neuropilin-1 protein
  

  Rat Neuropilin-1 (Phe22 - Ala810) Accession # Q9QWJ9	R	Rat Neuropilin-1 (Ser829 - Asp854) Accession # Q9QWJ9	IEGRDMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus					C-terminus

  
  R&D Systems rat Npn-1 may represent a previously unreported splice isoform of rat Npn-1.
• Accession #: Q9QWJ9
• N-terminal Sequence: Phe22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Nrp1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 119 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citrate Phosphate and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Neuropilin-1 Fc Chimera (NS0-expressed), CF

• BDCA4
• BDCA-4
• CD304 antigen
• CD304
• DKFZp686A03134
• DKFZp781F1414
• neuropilin 1
• Neuropilin1
• Neuropilin-1
• NP1
• NRP
• NRP1
• transmembrane receptor
• Vascular endothelial cell growth factor 165 receptor
• VEGF165R

Background

Neuropilin-1 (Npn-1, previously neuropilin; also named CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 922 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5 - 7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (8). Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the b1b2 region (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act asco-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both areco-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 
2. Gu, C. et al. (2003) Dev. Cell 5:45. 
3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
4. Soker, S. et al. (1998) Cell 92:735.
5. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 
6. Cackowski, F.C. et al. (2004) Genomics 84:82. 
7. Rossignol, M. et al. (2000) Genomics 70:211.
8. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
9. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
10. Yaron, A. et al. (2005) Neuron 45:513.
11. Narazaki, M. and G. Tosato (2006) Blood 107:3892.
12. Tordjman, R. et al. (2002) Nat. Immunol. 3477.

Publications for Neuropilin-1 (566-NNS)(3)

Publications using 566-NNS

• Uniewicz KA, Cross MJ, Fernig DG Exogenous recombinant dimeric neuropilin-1 is sufficient to drive angiogenesis. J. Biol. Chem., 2010-10-18;286(1):12-23. 2010-10-18 [PMID: 20956519] (Bioassay, Human)
• Narazaki M, Segarra M, Tosato G Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008-02-13;111(8):4126-36. 2008-02-13 [PMID: 18272814] (Binding Assay, Human)
• McLennan R, Kulesa PM In vivo analysis reveals a critical role for neuropilin-1 in cranial neural crest cell migration in chick. Dev. Biol., 2006-08-10;301(1):227-39. 2006-08-10 [PMID: 16959234] (In Vivo, Chicken)

FAQs for Neuropilin-1 (566-NNS). (Showing 1 - 2 of 2 FAQs).

1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
   • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
2. What research areas can Neuropilin-1 antibodies be used in?
   • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.

Bioinformatics

• Gene Symbol: Nrp1
• Uniprot:
  • Q9QWJ9()