Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF Summary

• Details of Functionality: Measured by its ability to inhibit Lymphotoxin alpha 1/ beta 2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756. The ED₅₀ for this effect is 0.15-0.9 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived rat Lymphotoxin beta R/TNFRSF3 protein
  

  Rat Lymphotoxin beta R (Gln29-Met222) Accession # NP_001008316	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: NP_001008316
• N-terminal Sequence: No results obtained. Gln29 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ltbr
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-68 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

• CD18
• D12S370
• ltbetar
• LT-beta-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

Lymphotoxin  beta R (LT beta R; also called TNFRSF3, TNF RIII,or TNF Rrp) is a type I transmembrane glycoprotein within the TNF receptor superfamily (1-3). The rat LT beta R cDNA encodes a 415 amino acid (aa) protein that includes a 30 aa signal peptide, a 191 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine-rich motifs characteristic of the TNF receptor superfamily (2). Within the ECD, rat LT beta R shares 90% and 66% aa sequence identity with the ECD of mouse and human LT beta R, respectively. Soluble LT beta R, which can be formed by proteolytic cleavage of the ECD, functions as a decoy receptor for LT beta R ligands and is involved in the suppression of autoimmunity (4, 5). LT beta R is constitutively expressed in cells of myeloid lineage and is upregulated during tissue regeneration (6). It is expressed on mesenchymal stromal organizing cells that give rise to primary, secondary, and tertiary lymphoid structures (7-9). Mice deficient in LT beta R fail to form secondary lymphoid structures (4). LT beta R ligands include homotrimers of LIGHT/TNFSF14 and the heterotrimeric Lymphotoxin alpha 1/ beta 2 (2). Ligand engagement of LT beta R has been shown to induce NF kappa B activation through canonical (IKK) or alternative (NIK/RelB) signaling pathways (5, 10). LT beta R signaling induces production of cytokines (TRANCE/RANK Ligand/TNFSF11, IL-7), chemokines (CXCL8/IL-8, CXCL13/BCL/BCA-1, CCL19/MIP-3 beta , CCL21/6Ckine, CCL2/MCP-1), and adhesion molecules (VCAM-1/CD106, ICAM-1/CD54, MAdCAM) (8, 11). LT beta R is involved in lipid metabolism, atherosclerosis, and intestinal epithelial homeostasis (4, 12, 13). It also regulates cell growth and can initiate inflammation-related carcinogenesis (5, 14).

1. Force, W.R. et al. (1995) J. Immunol. 155:5280.
2. Remouchamps, C. et al. (2011) Cytokine Growth Factor Rev. 22:301.
3. Crowe, P.D. et al. (1994) Science 264:707.
4. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
5. Wolf, M.J. et al. (2010) Oncogene 29:5006.
6. Ware, C.F. (2005) Annu. Rev. Immunol. 23:787.
7. Boulianne, B. et al. (2012) Front. Immunol. 3:243.
8. Mouri, Y. et al. (2011) J. Immunol. 186:5047.
9. van de Pavert, S.A. and R.E. Mebius (2010) Nat. Rev. Immunol. 10:664.
10. Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
11. Mikami, Y. et al. (2014) PLoS One 9:e114791.
12. Macho-Fernandez, E. et al. (2015) Mucosal Immunol. 8:403.
13. Browning, J.L. (2012) Mucosal Immunol. 5:228.
14. Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Bioinformatics

• Gene Symbol: Ltbr
• Uniprot:
  • NP_001008316()