Recombinant Rat LILRC2 Protein, CF

When Recombinant Rat LILRC2 (Catalog # 9808‑T4) is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914‑AN) binds with an ED50 of 0.04‑0.24 μg/mL.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat LILRC2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Rat LILRC2 is immobilized at 2 μg/mL,100 μL/well, the concentration of Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) that produces 50% of the optimal binding response is 0.04-0.24 μg/mL
• Source: Mouse myeloma cell line, NS0-derived rat LILRC2 protein
  Met1-Asn230, with a C-terminal 6-His tag
• Accession #: AAY44812
• N-terminal Sequence: Gly24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 32-43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat LILRC2 Protein, CF

• LILRC2

Background

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRC2 (leukocyte immunoglobulin-like receptor C2) is a 35 kDa member of the LILRs that are expressed on hematopoietic cells. Mature rat LILRC2 is a type I transmembrane (TM) protein that is 241 amino acids (aa) in length. It contains a 207 aa extracellular domain (ECD), a 19 aa TM segment, and a short cytoplasmic tail (2). The ECD contains two Ig-like domains while the TM segment possesses an Arg residue (2, 3). This suggests that LILRC2 is an activating receptor that may interact with Fc epsilon RI gamma and/or CD3 zeta. Cells expressing LILRC2 include neutrophils, B cells, and CD4⁺ and CD8⁺ T cells (2). No human or mouse LILRC2 is known. Rat LILRC2 shares 47.9% identity with LILRC1. R&D Systems in-house testing indicates that LILRC2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (4).

1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
2. Hoelsbrekken, S.E. et al. (2005) Immunogenetics 57:479.
3. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
4. Zhang, C.C. et al. (2012) Nature 485:656.

Bioinformatics

• Uniprot:
  • AAY44812()