Recombinant Rat IL-12 Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat IL-12 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.025-0.25 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat IL-12 protein
Rat IL-12 p40
(Met23 - Ser335)
Accession # NP_072133
6-His tag
Rat IL-12 p35
(Arg23 - Ser215)
Accession # Q9R103
6-His tag
N-terminus C-terminus
Accession #
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12a
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.8 kDa (p40), 22.6 kDa (p35).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
42-45 kDa and 26-28 kDa, reducing conditions
Publications
Read Publications using
1760-RL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-12 Protein

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines, which have important immunological functions. IL-12 is composed of two disulfide-linked subunits of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G‑CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain, and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12, the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%, and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40 contains 313 aa and can exist in multiple forms, including monomer, homodimer, heterodimer linked to p19 (forming IL-23), and heterodimer linked to p35 (forming IL-12). The expression of p40 is upregulated by substances such as LPS and CpG that activate antigen-presenting cells. Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40, respectively. Cells known to produce IL-12 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two type I transmembrane proteins: a 100 kDa ligand-binding subunit (IL‑12 R beta 1) and a 130 kDa signal transducing subunit (IL‑12 R beta 2). IL‑12 facilitates hematopoietic stem cell proliferation, induces NK cell proliferation, and potentiates the expansion and late activation of Th1 CD4+ T cells (1 - 4).

  1. Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Brombacher, F. et al. (2003) Trends Immunol. 24:207.
  4. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.

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Publications for IL-12 (1760-RL)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, Multiplex Assay.


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Multiplex Assay
(1)
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