Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, Recombinant Rat Fc gamma RIIIA/CD16a (Catalog # 9596-CD) binds with an ED50 of 0.1-0.6 μg/mL.

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Rat Fc gamma RIIIA/CD16a that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived rat Fc gamma RIIIA/CD16a protein
  Gly21-Pro200, with a C-ternimal 6-His tag
• Accession #: NP_997486
• N-terminal Sequence: Ala20 and Gly21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 24-36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

• CD16a antigen
• CD16a
• CD16FCRIIIA
• Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
• Fc fragment of IgG, low affinity IIIa, receptor for (CD16)
• Fc gamma receptor III-A
• Fc gamma RIIIA
• FCG3
• Fc-gamma receptor III-2 (CD 16)
• Fc-gamma receptor IIIb (CD16)
• Fc-gamma RIII
• Fc-gamma RIIIa
• Fc-gamma RIII-alpha
• FCGR3
• FCGR3A
• FCGRIII
• FcgRIIIA
• FcR-10
• FCRIII
• FcRIIIa
• IGFR3FcgammaRIIIA
• immunoglobulin G Fc receptor III
• low affinity III, receptor for (CD16)
• low affinity immunoglobulin gamma Fc region receptor III-A
• neutrophil-specific antigen NA

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). Fc gamma RIII has two isoforms, Fc gamma  RIIIA and Fc gamma  RIIIB. Fc gamma  RIII, the only Fc gamma  R found on natural killer cells, is also expressed on macrophages, neutrophils, and mast cells (1-3). Unlike Fc gamma  RI which binds IgG with high affinity, Fc gamma  RIIIA is a low affinity receptor that recognizes IgG as aggregates surrounding multivalent antigens during late immune responses (4, 5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma  RIIIA shares 83% and 63% amino acid sequence identity with mouse and human Fc gamma  RIIIA, respectively.

1. Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.

FAQs for Fc gamma RIIIA/CD16a (9596-CD). (Showing 1 - 1 of 1 FAQ).

1. I am interested in developing a surrogate ADCC assay (FC gamma III a binding assay) for which, I would require a CD16 Recombinant Protein as an antigen and Anti-Human IgG (Fc specific) as a secondary antibody as I am planning to perform this assay in an ELISA format. With this brief description, could you please suggest me the right CD16 Recombinant Protein among the three listed products in your web site (www.novusbio.com/proteins/cd16). Appreciate if you can share a reference article, where the use of this protein is cited.
   • The right CD16 protein for you depends on what sort of functionality you require for your assay. H00002214-P01 is made by a Taiwanese company called Abnova and we distribute it for them. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots and ELISA as a positive control with their antibody. NBP1-98908 is produced in E. coli and contains a His-tag and it also not guaranteed for functionality but may be due to its E. coli expression system. H00002214-G01 is also an Abnova and is made in their cell-free wheat germ expression system with proprietary liposome technology. This is a recently developed system by them and is intended to help the proteins fold and maintain functionality. Which protein would be best for you depends on what you require for your assay. Here is a link to our antibodies to the Fc region of human IgG. The best antibody for you will depend on what you would like to use for detection.

Bioinformatics

• Uniprot:
  • NP_997486()