Recombinant Rat EGF Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat EGF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 10-60 pg/mL.
• Source: E. coli-derived rat EGF protein
  Asn974-Arg1026, with an N-terminal Met
• Accession #: P07522
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Egf
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 6.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat EGF Protein, CF

• beta-urogastrone
• EGF
• epidermal growth factor (beta-urogastrone)
• epidermal growth factor
• hEGF
• HOMG4
• pro-epidermal growth factor
• URG
• Urogastrone

Background

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
3. Saggi, S.J. et al. (1992) DNA Cell Biol. 11:481.
4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Publications for EGF (3214-EG/CF)(12)

Publications using 3214-EG/CF

• Z Berkova, K Zacharovov, A Patikova, I Leontovyc, Z Hladikova, D Cerveny, E Tihlarikov, V Nedela, P Girman, D Jirak, F Saudek Decellularized Pancreatic Tail as Matrix for Pancreatic Islet Transplantation into the Greater Omentum in Rats Journal of functional biomaterials, 2022-09-30;13(4):. 2022-09-30 [PMID: 36278640] (Cell Culture, Rat)
• A López-Márq, C Carrasco-L, A Martínez-C, P Lemoine, CE Pierreux, P Santisteba Sox9 is involved in the thyroid differentiation program and is regulated by crosstalk between TSH, TGFbeta and thyroid transcription factors Scientific Reports, 2022-02-09;12(1):2144. 2022-02-09 [PMID: 35140269] (Bioassay, Rat)
• LT Frasson, B Dalmaso, PS Akamine, ET Kimura, DE Hamassaki, CB Del Debbio Let-7, Lin28 and Hmga2 Expression in Ciliary Epithelium and Retinal Progenitor Cells Investigative Ophthalmology & Visual Science, 2021-03-01;62(3):31. 2021-03-01 [PMID: 33749722] (Bioassay, Rat)
• B Fan, W Pan, X Wang, M Wei, A He, A Zhao, M Chopp, ZG Zhang, XS Liu Long noncoding RNA mediates stroke-induced neurogenesis Stem Cells, 2020-05-02;0(0):. 2020-05-02 [PMID: 32346940] (Bioassay, Rat)
• M Nakamura, H Nishida, K Yoshizaki, H Akiyoshi, S Hatoya, K Sugiura, T Inaba Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells J. Vet. Med. Sci., 2020-04-06;0(0):. 2020-04-06 [PMID: 32249241] (Cell Culture, Rat)
• W Zhang, X Li, S Sun, X Zhang Implantation of engineered conduction tissue in the rat heart Mol Med Rep, 2019-02-05;0(0):. 2019-02-05 [PMID: 30720107] (Bioassay, Rat)
• D Stöckmann, T Spannbruck, N Ale-Agha, P Jakobs, C Goy, N Dyballa-Ru, T Hornstein, A Kümper, A Kraegeloh, J Haendeler, K Unfried Non-Canonical Activation of the Epidermal Growth Factor Receptor by Carbon Nanoparticles Nanomaterials (Basel), 2018-04-23;8(4):. 2018-04-23 [PMID: 29690640] (Bioassay, Rat)
• M Zhang, MH Jiang, DW Kim, W Ahn, E Chung, Y Son, G Chi Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Na�ve Schwann Cells in the Sciatic Nerve Injury Model Biomed Res Int, 2017-06-20;2017(0):1252851. 2017-06-20 [PMID: 28713821] (Bioassay, Rat)
• O Stojadinov, TC Wikramanay, AC Villasante, NC Yin, L Liang, E Hinde, J Escandon, M Tomic-Cani, DM Ansell, R Paus, JJ Jimenez Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling Heliyon, 2017-05-30;3(5):e00309. 2017-05-30 [PMID: 28607955] (In Vivo, Rat)
• Kandasamy M, Lehner B, Kraus S, Sander P, Marschallinger J, Rivera F, Trumbach D, Ueberham U, Reitsamer H, Strauss O, Bogdahn U, Couillard-Despres S, Aigner L TGF-beta signalling in the adult neurogenic niche promotes stem cell quiescence as well as generation of new neurons. J Cell Mol Med, 2014-04-30;18(7):1444-59. 2014-04-30 [PMID: 24779367] (Bioassay, Rat)
• Matsumoto , Shinji, Fujii , Shinsuke, Sato , Akira, Ibuka , Souji, Kagawa , Yoshinor, Ishii , Masaru, Kikuchi , Akira A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures. EMBO J, 2014-02-20;33(7):702-18. 2014-02-20 [PMID: 24562386] (Bioassay, Rat)
• Gilbert RE, Huang Q, Thai K, Advani SL, Lee K, Yuen DA, Connelly KA, Advani A Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor. Kidney Int., 2011-03-09;79(12):1312-21. 2011-03-09 [PMID: 21389970] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: Egf
• Uniprot:
  • P07522()