Recombinant Rat Dkk-1 Protein

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Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010‑DK) inhibits a constant dose of ...read more

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Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat Dkk-1 Protein Summary

Details of Functionality
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ND50 for this effect is 0.1‑0.5 µg/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Rat Dkk-1 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). The ED50 for this effect is 50-300 ng/mL.
Source
Mouse myeloma cell line, NS0-derived rat Dkk-1 protein
Ser23-His270, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser23
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
27.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38-42 kDa, reducing conditions
Publications
Read Publications using
4010-DK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Dkk-1 Protein

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature rat Dkk-1 is a 40 kDa glycosylated protein that shows 96%, 86% and 82% aa sequence identity with mouse, human and cow Dkk-1, respectively. It also shows 41% and 34% aa identity with rat Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 10). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Neuronal expression appears to be required for ischemic neuronal death (11).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  11. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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Publications for Dkk-1 (4010-DK)(5)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioassay.


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