Recombinant Mouse Wnt-3a Protein

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Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. The ED50 for this effect is ≤5 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse Wnt-3a Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. Fischer, L. et al. (2002) J. Biol. Chem. 277:30870. The ED50 for this effect is ≤5 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.

Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line.
Optimal concentrations should be determined by each laboratory for each application.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-3a protein
Ser19-Lys352
Accession #
N-terminal Sequence
Ser19
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt3a
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41 kDa, reducing conditions
Publications
Read Publications using
1324-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 40 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-3a Protein

  • MGC119418
  • MGC119419
  • MGC119420
  • protein Wnt-3a
  • wingless-type MMTV integration site family, member 3A
  • Wnt3a
  • Wnt-3a

Background

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes (1). Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). As intracellular beta -catenin levels rise, beta -catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (3). Endo-IWR 1 (Catalog # 3532, # PSM1324) is a cell-permeant small molecule inhibitor of Axin turnover that suppresses Wnt signal transduction by stabilizing the beta -catenin destruction complex (4). Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues (5). Wnt-3a has two N-linked glycosylation sites (Asn 87, Asn 298), and Ser 209 is modified with palmitoleic acid (6). Glycosylation and acylation are essential for efficient Wnt secretion and biological activity, respectively (6, 7). Mouse Wnt-3a shares 96% amino acid (aa) identity with human Wnt-3a, and 97% with bovine and canine Wnt-3a. The rat Wnt-3a precursor shares 100% aa identity with mouse Wnt-3a aa 63-352 (8), and also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation (9-12). Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells (13-15).
  1. Willert, K. and Nusse, R. (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  3. Korinek, V. et al. (1997) Science 275:1784.
  4. Chen, B. et al. (2009) Nat. Chem. Biol. 5:100.
  5. Smolich, B.D. et al. (1993) Mol. Biol. Cell 4:1267.
  6. Takada, R. et al. (2006) Dev. Cell 11:791.
  7. Komekado, H. (2007) Genes Cells 12:521.
  8. Entrez Accession # NP_001100475.
  9. Dunty Jr. W. C. et al. (2008) Development 135:85.
  10. Ikeya, M. and S. Takada (2001) Mech. Dev. 103:27.
  11. Lee, S. M. et al. (2000) Development 127:457.
  12. Takada, S. et al. (1994) Genes Dev. 8:174.
  13. Willert, K. et al. (2003) Nature 423:6938.
  14. Kalani, M.Y. et al. (2008) Proc. Natl. Acad. Sci. USA 105:16970.
  15. Ten Berge, D. et al. (2011) Nat. Cell Biol. 13:1070.

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Publications for Wnt-3a (1324-WN)(137)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Rabbit.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, In Vivo, Organoid Culture.


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Binding Assay
(1)
Bioassay
(121)
Cell Culture
(2)
In Vivo
(7)
Organoid Culture
(1)
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Human
(25)
Mouse
(103)
Rat
(3)
Chicken
(3)
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(1)
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Showing Publications 1 - 10 of 137. Show All 137 Publications.
Publications using 1324-WN Applications Species
KL VanDussen, NM Sonnek, TS Stappenbec L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams Stem Cell Res, 2019;37(0):101430. 2019 [PMID: 30933720] (Bioassay, Human) Bioassay Human
P Shooshtari, A Helness, C Vadnais, N Brouwer, H Beauchemin, R Chen, H Bagci, FJT Staal, JF Coté, T Möröy Gfi1b regulates the level of Wnt/?-catenin signaling in hematopoietic stem cells and megakaryocytes Nat Commun, 2019;10(1):1270. 2019 [PMID: 30894540] (Bioassay, Mouse) Bioassay Mouse
M Chapellier, P Peña-Martí, R Ramakrishn, M Eriksson, M Safaee-Tal, C Orsmark-Pi, H Lilljebjör, C Högberg, A Hagström-A, T Fioretos, J Larsson, M Järås Arrayed molecular barcoding identifies TNFSF13 as a positive regulator of acute myeloid leukemia-initiating cells Haematologica, 2019;0(0):. 2019 [PMID: 30819903] (Bioassay, Mouse) Bioassay Mouse
J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects Cell Rep, 2019;26(9):2494-2508.e7. 2019 [PMID: 30811996]
L Qian, H Zhang, Y Gu, D Li, S He, H Wang, Y Cheng, W Yang, H Yu, X Zhao, W Cai, L Meng, M Jin, Y Wang, Y Zhang Reduced production of laminin by hepatic stellate cells contributes to impairment in oval cell response to liver injury in aged mice Aging (Albany NY), 2018;0(0):. 2018 [PMID: 30513510] (Bioassay, Mouse) Bioassay Mouse
YB Hu, C Yan, L Mu, YL Mi, H Zhao, H Hu, XL Li, DD Tao, YQ Wu, JP Gong, JC Qin Exosomal Wnt-induced dedifferentiation of colorectal cancer cells contributes to chemotherapy resistance Oncogene, 2018;0(0):. 2018 [PMID: 30390075] (Bioassay, Human) Bioassay Human
S Olabi, A Ucar, K Brennan, CH Streuli Integrin-Rac signalling for mammary epithelial stem cell self-renewal Breast Cancer Res., 2018;20(1):128. 2018 [PMID: 30348189] (Bioassay, Mouse) Bioassay Mouse
A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids Elife, 2018;7(0):. 2018 [PMID: 30311909] (Bioassay, Human) Bioassay Human
Y Xie, ES Park, D Xiang, Z Li Long-term organoid culture reveals enrichment of organoid-forming epithelial cells in the fimbrial portion of mouse fallopian tube Stem Cell Res, 2018;32(0):51-60. 2018 [PMID: 30176443] (Bioassay, Mouse) Bioassay Mouse
A Spangler, EY Su, AM Craft, P Cahan A single cell transcriptional portrait of embryoid body differentiation and comparison to progenitors of the developing embryo Stem Cell Res, 2018;31(0):201-215. 2018 [PMID: 30118958] (Bioassay, Mouse) Bioassay Mouse
Show All 137 Publications.

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Bioinformatics

Gene Symbol Wnt3a
Uniprot