236-EG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the
human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (
MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (
AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (
5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (
5748; blue)." class="big_lightbox">

236-EG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the
human intestinal organoid culture protocol. (A) Human intestinal organoids were stained using a Rat Anti-Human/Mouse/Rat Vimentin Monoclonal Antibody (
MAB2105; green) and a Goat Anti-Human/Mouse Desmin Antigen Affinity-purified Polyclonal Antibody (
AF3844; red) to visualize myofibroblast cells and counterstained with DAPI (
5748; blue). (B) Human intestinal organoids were stained using a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; green) and a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-44431; red) and counterstained with DAPI (
5748; blue)." class="big_thumb" />
236-EG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the
human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; red) and DAPI (
5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (
MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; red) and DAPI (
5748; blue). The image shown was taken at 20x magnification." class="big_lightbox">

236-EG), Recombinant Human Noggin (
6057-NG), and Recombinant Human R-Spondin 1 (
4645-RS), along with the other reagents listed in the intestinal organoid culture medium recipe in the
human intestinal organoid culture protocol. (A) Organoids were fixed and stained with a Mouse Anti-Human MUC2 Monoclonal Antibody (Novus Biologicals; Catalog # NBP2-44431; green) to visualize intestinal goblet cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; red) and DAPI (
5748; blue). The image shown was taken at 10x magnification. (B) Organoids were fixed and stained with a Mouse Anti-Human Chromogranin A Monoclonal Antibody (
MAB90981; green) to visualize enteroendocrine cells and counterstained with a Goat Anti-Human/Mouse E-Cadherin Antigen Affinity-purified Polyclonal Antibody (
AF748; red) and DAPI (
5748; blue). The image shown was taken at 20x magnification." class="big_thumb" />