Recombinant Mouse Wnt-10b Protein, CF

Recombinant Mouse Wnt-10b (Catalog # 2110-WN/CF) stimulates cell proliferation/survival of the IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is 0.3-1.2 μg/mL.

1 μg/lane of Recombinant Mouse Wnt-10b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 42 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Wnt-10b Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED₅₀ for this effect is 0.3-1.2 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-10b protein
  Met1-Lys389
• Accession #: NP_035848
• N-terminal Sequence: Asn29
• Protein/Peptide Type: Recombinant Proteins
• Gene: Wnt10b
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-10b Protein, CF

• protein Wnt-10b
• Protein Wnt-12
• SHFM6WNT-10B protein
• wingless-type MMTV integration site family, member 10B
• Wnt10b
• Wnt-10b
• WNT12
• Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Mouse Wnt-10b cDNA encodes a 361 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143‑238 of the precursor (1, 4). Mouse Wnt-10b shares 97‑98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis‑sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8⁺ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15).

1. Wang, J. and G.M. Shackleford (1996) Oncogene 13:1537.
2. Katoh, M. (2008) Curr. Drug Targets 9:565.
3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
4. Swiss Prot Accession # P48614.
5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
8. Wright, W.S. et al. (2007) Diabetes 56:295.
9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
15. Cynthia K. et al. (2007) Proc. Natl. Acad. Sci. USA.

Publications for Wnt-10b (2110-WN/CF)(4)

Publications using 2110-WN/CF

• Y Ouji, M Misu, T Kitamura, D Okuzaki, M Yoshikawa Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture Scientific Reports, 2022-06-30;12(1):11011. 2022-06-30 [PMID: 35773408] (Bioassay, Mouse)
• A Reggio, M Rosina, A Palma, A Cerquone P, LL Petrilli, C Gargioli, C Fuoco, E Micarelli, G Giuliani, M Cerretani, A Bresciani, F Sacco, L Castagnoli, G Cesareni Adipogenesis of skeletal muscle fibro/adipogenic progenitors is affected by the WNT5a/GSK3/beta-catenin axis Cell Death Differ., 2020-05-07;0(0):. 2020-05-07 [PMID: 32382110] (Bioassay, Mouse)
• CE Macdougall, EG Wood, J Loschko, V Scagliotti, FC Cassidy, ME Robinson, N Feldhahn, L Castellano, MB Voisin, F Marelli-Be, C Gaston-Mas, M Charalambo, MP Longhi Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets Cell Metab., 2018-03-06;27(3):588-601.e4. 2018-03-06 [PMID: 29514067] (Bioassay, Mouse)
• Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 2014-03-27;4(0):4493. 2014-03-27 [PMID: 24670389] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Wnt10b
• Uniprot:
  • NP_035848()