Recombinant Mouse Wnt-10b Protein

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1 μg/lane of Recombinant Mouse Wnt-10b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 42 kDa.
Recombinant Mouse Wnt-10b (Catalog # 2110-WN) stimulates cell proliferation/survival of the IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is 0.3-1.2 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse Wnt-10b Protein Summary

Details of Functionality
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.3-1.2 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-10b protein
Met1-Lys389
Accession #
N-terminal Sequence
Asn29
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt10b
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
40.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
42 kDa, reducing conditions
Publications
Read Publication using
2110-WN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-10b Protein

  • protein Wnt-10b
  • Protein Wnt-12
  • SHFM6WNT-10B protein
  • wingless-type MMTV integration site family, member 10B
  • Wnt10b
  • Wnt-10b
  • WNT12
  • Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Mouse Wnt-10b cDNA encodes a 361 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143‑238 of the precursor (1, 4). Mouse Wnt-10b shares 97‑98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis‑sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15).

  1. Wang, J. and G.M. Shackleford (1996) Oncogene 13:1537.
  2. Katoh, M. (2008) Curr. Drug Targets 9:565.
  3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
  4. Swiss Prot Accession # P48614.
  5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
  6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
  7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
  8. Wright, W.S. et al. (2007) Diabetes 56:295.
  9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
  10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
  11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
  12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
  13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
  14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
  15. Cynthia K. et al. (2007) Proc. Natl. Acad. Sci. USA.

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Publications for Wnt-10b (2110-WN)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Wnt10b
Entrez
Uniprot