Recombinant Mouse Wnt-10b Protein


1 μg/lane of Recombinant Mouse Wnt-10b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 42 kDa.
Recombinant Mouse Wnt-10b (Catalog # 2110-WN) stimulates cell proliferation/survival of the IEC-18 rat small intestinal epithelial cell line. The ED50 for this effect is 0.3-1.2 μg/mL.

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Applications Bioactivity

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Recombinant Mouse Wnt-10b Protein Summary

Details of Functionality
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.3-1.2 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-10b protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
40.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
42 kDa, reducing conditions
Read Publication using
2110-WN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS with BSA as a carrier protein.
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-10b Protein

  • protein Wnt-10b
  • Protein Wnt-12
  • SHFM6WNT-10B protein
  • wingless-type MMTV integration site family, member 10B
  • Wnt10b
  • Wnt-10b
  • WNT12
  • Wnt-12


Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Mouse Wnt-10b cDNA encodes a 361 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143‑238 of the precursor (1, 4). Mouse Wnt-10b shares 97‑98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis‑sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15).

  1. Wang, J. and G.M. Shackleford (1996) Oncogene 13:1537.
  2. Katoh, M. (2008) Curr. Drug Targets 9:565.
  3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
  4. Swiss Prot Accession # P48614.
  5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
  6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
  7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
  8. Wright, W.S. et al. (2007) Diabetes 56:295.
  9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
  10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
  11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
  12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
  13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
  14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
  15. Cynthia K. et al. (2007) Proc. Natl. Acad. Sci. USA.

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Publications for Wnt-10b (2110-WN)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.

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Gene Symbol Wnt10b