Wnt-9a Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Wnt-9a Antibody Summary

Immunogen
E. coli-derived recombinant human Wnt‑9a
Pro48-Glu92 & Arg231-Phe304
Accession # O14904.2
Specificity
Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑9b is observed and less than 1% cross-reactivity with rmWnt-1 and rmWnt-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WNT9A
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF3157 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-9a Antibody

  • MGC138165
  • MGC141991
  • Protein Wnt-14
  • wingless-type MMTV integration site family, member 14
  • wingless-type MMTV integration site family, member 9A
  • Wnt-14
  • WNT14protein Wnt-9a
  • Wnt9a
  • Wnt-9a

Background

Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for Frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic, and breast cancer cells. Secretion of Wnt-9a is believed to play a role in avian atrioventricular cardiac cushion development. Human Wnt-9a is 98% amino acid (aa) identical with both mouse and rat Wnt-9a.

The Wnts make up a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt family members vary in length between 350 and 400 aa, possess 22 to 24 conserved cysteines, are highly hydrophobic, and show 20 - 85% aa identity within the Wnt family. In general, there are three signaling pathways associated with Wnt-receptor interaction. The first is commonly called the canonical pathway and ultimately culminates in beta-Catenin accumulation and TCF/LEF-1-mediated gene transcription. The other two less well-defined non-canonical pathways include the Wnt/Ca2+ pathway and the planar cell polarity (PCP) pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Wnt-9a Antibody (AF3157)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Neut, WB.


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Neut
(1)
WB
(1)
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Human
(2)
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Video Protocols

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Bioinformatics

Gene Symbol WNT9A
Entrez
Uniprot