Wnt-9a Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Wnt-9a Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Wnt‑9a
  Pro48-Glu92 & Arg231-Phe304
  Accession # O14904.2
• Specificity: Detects human Wnt-9a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑9b is observed and less than 1% cross-reactivity with rmWnt-1 and rmWnt-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: WNT9A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-9a Antibody [Unconjugated]

• MGC138165
• MGC141991
• Protein Wnt-14
• wingless-type MMTV integration site family, member 14
• wingless-type MMTV integration site family, member 9A
• Wnt-14
• WNT14protein Wnt-9a
• Wnt9a
• Wnt-9a

Background

Wnt-9a, previously known as Wnt-14, is a secreted glycoprotein belonging to the Wnt family of signaling molecules that are important in development. Within the sequences used as immunogens, human Wnt-9a shares 97% amino acid sequence identity with the mouse or rat protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Wnt-9a Antibody (AF3157)(2)

Publications using AF3157

• Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-07-03;30(1):163-72. 2015-07-03 [PMID: 26139429] (Neutralization, Human)
• Chigita S, Sugiura T, Abe M, Kobayashi Y, Shimoda M, Onoda M, Shirasuna K CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells. Int J Oncol, 2012-10-17;41(6):2021-8. 2012-10-17 [PMID: 23076981] (Western Blot, Human)

Bioinformatics

• Gene Symbol: WNT9A
• Uniprot:
  • O14904.2()