Recombinant Mouse Wnt-9b Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Wnt-9b Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The typical ED50 range is 100-400 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-9b protein
Ala24-Arg359
Accession #
N-terminal Sequence
Ala24
Protein/Peptide Type
Recombinant Proteins
Gene
Wnt9b
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
39-41 kDa, reducing conditions
Publications
Read Publications using
3669-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, EDTA and CHAPS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Wnt-9b Protein, CF

  • Protein Wnt-14b
  • wingless-type MMTV integration site family, member 15
  • wingless-type MMTV integration site family, member 9B
  • Wnt-14b
  • WNT14Bprotein Wnt-9b
  • Wnt-15
  • WNT15Protein Wnt-15
  • Wnt9b
  • Wnt-9b

Background

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1-3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).
  1. www.stanford.edu/~rnusse/wntwindow.html
  2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
  4. Qian, J. et al. (2003) Genomics 81:34.
  5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
  6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
  7. Park, J-S. et al. (2007) Development 134:2533.
  8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
  9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

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3669-WN/CF
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Publications for Wnt-9b (3669-WN/CF)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Binding Assay, Bioassay, TOPflash assay.


Filter By Application
Binding Assay
(2)
Bioassay
(4)
TOPflash assay
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
All Species
Showing Publications 1 - 7 of 7.
Publications using 3669-WN/CF Applications Species
KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019;14(4):e0215139. 2019 [PMID: 30978219] (TOPflash assay, Mouse) TOPflash assay Mouse
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 2014;4(0):4493. 2014 [PMID: 24670389] (Bioassay, Mouse) Bioassay Mouse
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human
Goel S, Chin EN, Fakhraldeen SA Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J. Biol. Chem., 2012;287(20):16454-66. 2012 [PMID: 22433869] (Bioassay, Mouse) Bioassay Mouse
Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida VI, Liu BY, Phamluong K, Cao TC, Carano RA, Ernst JA, Solloway M, Rubinfeld B, Hannoush RN, Wu Y, Polakis P, Costa M Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies. PLoS ONE, 2010;5(9):e12682. 2010 [PMID: 20856934] (Binding Assay, Human) Binding Assay Human
Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J. Biol. Chem., 2010;285(12):9172-9. 2010 [PMID: 20093360] (Binding Assay, Human) Binding Assay Human

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Bioinformatics

Gene Symbol Wnt9b
Uniprot