Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

When Recombinant Mouse Tyro3/Dtk His-tag Protein is immobilized at 1 μg/mL (100 μL/well), Recombinant Mouse GAS6 (Full Length) Protein (Catalog # 8310-GS) binds with an ED50 of 0.4‑2.4 μg/mL.

2 μg/lane of Recombinant Mouse Tyro3/Dtk His-tag Protein (Catalog # 10480-DT) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Tyro3/Dtk His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Tyro3/Dtk His-tag (Catalog # 10480-DT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Gas6 (Catalog # 8310-GS) binds with an ED₅₀ of 0.4-2.4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Tyro3/Dtk protein
  Ala31-Thr418, with a C-terminal 6-His tag
• Accession #: P55144.2
• N-terminal Sequence: Ala31
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-75 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Tyro3/Dtk His-tag Protein, CF

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. Mouse DTK is a 42kDa protein with a 30 amino acid (aa) signal sequence, 389 aa extracellular domain (ECD), and a 20 aa transmembrane segment. The ECD of mouse DTK shares 85% and 96% sequence identity with the ECD of human and rat respectively. The extracellular domain of these proteins contains two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain. In embryoid stem cells growing in the presence of leukemia inhibitory factor, DTK is abundantly expressed and this level of expression is maintained in differentiating embryoid stem cells and cystic embryoid bodies. In mid-gestational embryos, DTK RNA is expressed in many tissues including brain, eye, thymus, lung, heart, gut, liver, testis and limbs. In contrast, expression of DTK in adult mice becomes restricted to brain, portions of gastrointestinal tract, bladder, testis and ovary.  Overexpression of DTK and several other receptor tyrosine kinase have been found in small cell lung cancer (4).

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
3. Crosier, P. et al. (1994) Growth Factors 11:125.
4. Hamilton, G. et al. (2015) Oncoscience 2:629.

Bioinformatics

• Uniprot:
  • P55144.2()