Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 0.5-6 µg/mL in the presence of 1 µg/mL recombinant mouse TWEAK.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse TWEAK R/TNFRSF12 protein
  

  Mouse TWEAK R (Glu28-Trp79) Accession # Q9CR75	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9CR75
• N-terminal Sequence: Glu28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfrsf12a
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 32 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera Protein, CF

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2-6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
2. Feng, S. et al. (2000) Am. J. Pathol. 156:1253.
3. Wiley, S. et al. (2001) Immunity 15:837.
4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Publications for TWEAK R/TNFRSF12 (1610-TW)(2)

Publications using 1610-TW

• Ameri H, Liu H, Liu R, Ha Y, Paulucci-Holthauzen A, Hu S, Motamedi M, Godley B, Tilton R, Zhang W TWEAK/Fn14 pathway is a novel mediator of retinal neovascularization. Invest Ophthalmol Vis Sci, 2014-02-10;55(2):801-13. 2014-02-10 [PMID: 24408972] (In Vivo, Mouse)
• Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells. Arthritis Res. Ther., 2006-01-01;8(5):R146. 2006-01-01 [PMID: 16945157] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Tnfrsf12a
• Uniprot:
  • Q9CR75()