Recombinant Mouse TRAIL/TNFSF10 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse TRAIL/TNFSF10 Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
E. coli-derived mouse TRAIL/TNFSF10 protein
Pro118-Asn291
Accession #
N-terminal Sequence
Pro118
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfsf10
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kda.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1121-TL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, Ammonium Sulfate and ZnSO4 with Trehalose and with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS. 

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse TRAIL/TNFSF10 Protein

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

TNF-related apoptosis-inducing ligand (TRAIL), also called apoptosis 2 ligand (Apo2L) for its similarity in sequence, structure, and function to Fas Ligand/Apo1L, is a 33-35 kDa type II transmembrane glycoprotein of the tumor necrosis factor superfamily, designated TNFSF10 (1‑3). Mouse TRAIL cDNA encodes a 17 amino acid (aa) N-terminal intracellular domain, a 20 aa transmembrane domain and a 253 aa extracellular domain. Like most TNF family members, TRAIL is bioactive as a homotrimer (1). Unlike other TNF family members, a zinc ion complexed by human Cys 230 (mouse Cys 240) of each of the three monomers is critical for structural stability (4, 5). Either transmembrane or cysteine protease-released soluble sTRAIL induce apoptosis of many transformed cell lines, but rarely of normal cells (3, 6). Accordingly, TRAIL is suggested to have a role in tumor surveillance (1). Mice with genetically disrupted TRAIL have defective thymocyte apoptosis, creating faulty negative selection and some increased susceptibility to induced autoimmune diseases (7). In humans, TRAIL controls apoptosis of erythrocyte precursors and sTRAIL is inversely correlated with hemoglobin (1, 8). TRAIL transcripts are constitutively expressed in a variety of human (and presumably mouse) tissues and mononuclear cells (2, 3). Only one of two receptors that transduce apoptotic signals in humans is found in the mouse (TRAIL R2/DR5 but not TRAIL R1/DR4) (1). Mice express TRAIL receptors DcTRAIL R1/TNFRSF23 and DcTRAIL R2/TNFRSF22. These receptors lack death domains, but differ in structure from human regulatory receptors TRAIL R3 and TRAIL R4 (9). Osteoprotegerin has been identified in humans as a TRAIL receptor, but binding in mouse has not yet been demonstrated (1, 10). Mouse TRAIL shows 85% aa identity with rat TRAIL and 70% aa identity with human, bovine, and porcine TRAIL within the TNF homology domain (aa 118-291).

  1. Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Bodmer, J.L. et al. (2000) J. Biol. Chem. 275:20632.
  5. Hymowitz, S.G. et al. (2000) Biochemistry 39:633.
  6. Sedger, L. M. et al. (2002) Eur. J. Immunol. 32:2246.
  7. Lamhamedi-Cherradi, S.E. et al. (2003) Nat. Immunol. 4:255.
  8. Choi, J.W. (2005) Ann. Hematol. 84:728.
  9. Schneider, P. et al. (2003) J. Biol. Chem. 278:5444.
  10. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

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1121-TL
Species: Mu
Applications: Bioactivity

Publications for TRAIL/TNFSF10 (1121-TL)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, In Vivo, In vivo.


Filter By Application
Bioassay
(4)
In Vivo
(2)
In vivo
(1)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using 1121-TL Applications Species
S Tian, DH Liu, D Wang, F Ren, P Xia Aldehyde Dehydrogenase 1 (ALDH1) Promotes the Toxicity of TRAIL in Non-Small Cell Lung Cancer Cells via Post-Transcriptional Regulation of MEK-1 Expression Cell. Physiol. Biochem., 2018;51(1):217-227. 2018 [PMID: 30448845] (In Vivo, Mouse) In Vivo Mouse
Su, Tian, Bondar, Tanya, Zhou, Xu, Zhang, Cuiling, He, Hang, Medzhitov, Ruslan Two-signal requirement for growth-promoting function of Yap in hepatocytes. Elife, 2015;4(0):. 2015 [PMID: 25667983] (Bioassay, Mouse) Bioassay Mouse
Kichev A, Rousset C, Baburamani A, Levison S, Wood T, Gressens P, Thornton C, Hagberg H Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) signaling and cell death in the immature central nervous system after hypoxia-ischemia and inflammation. J Biol Chem, 2014;289(13):9430-9. 2014 [PMID: 24509861] (Bioassay, Mouse) Bioassay Mouse
Starkey M, Nguyen D, Essilfie A, Kim R, Hatchwell L, Collison A, Yagita H, Foster P, Horvat J, Mattes J, Hansbro P Tumor necrosis factor-related apoptosis-inducing ligand translates neonatal respiratory infection into chronic lung disease. Mucosal Immunol, 2014;7(3):478-88. 2014 [PMID: 24045576] (In vivo, Mouse) In vivo Mouse
Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012;109(25):9971-6. 2012 [PMID: 22665806] (Bioassay, Mouse) Bioassay Mouse
Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009;384(3):296-300. 2009 [PMID: 19406102] (In Vivo, Mouse) In Vivo Mouse
Dumitru CA, Carpinteiro A, Trarbach T, Hengge UR, Gulbins E Doxorubicin enhances TRAIL-induced cell death via ceramide-enriched membrane platforms. Apoptosis, 2007;12(8):1533-41. 2007 [PMID: 17520194] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Tnfsf10
Uniprot