Recombinant Mouse SR-BI Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SR-BI Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED₅₀ for this effect is 0.4-2 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse SR-BI protein
  

  Mouse SR-BI (Pro33-Tyr443) Accession # Q61009-1	IEGRMDP	Mouse IgG2a (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: Q61009-1
• N-terminal Sequence: Pro33
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SR-BI Fc Chimera Protein, CF

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

Scavenger Receptor, class B, member 1 (SR-BI), gene name SCARB1, is also known as CD36L1 (CD36-like 1) or CLA-1 (CD36 and LIMPII analogous 1) (1-5). SR-BI is a transmembrane glycoprotein found on macrophages, liver cells and other steroidogenic cells as a lipoprotein receptor. The 509 amino acid (aa) mouse SR-BI contains a central extracellular domain (ECD), flanked by N- and C-terminal transmembrane domains. A mouse splice variant differs at the C-terminal cytoplasmic domain (SR-BII, 506 aa) (2). The mouse SR-BI ECD shares 81% and 92% aa sequence identity with human and rat SR-BI, respectively. SR-BI functions in reverse cholesterol transport (RCT), which is thought to be anti-atherogenic by facilitating transport of cholesteryl esters from macrophages back to the liver for degradation (3). In rodent hepatocytes, SR-BI is the main receptor mediating RCT, while human hepatocytes also express a second mediator, CETP (cholesteryl ester transfer protein) (3-5). On endothelial cells, signaling through SR-BI activates nitric oxide production, which attenuates monocyte adhesion (6). On adrenocortical cells, SR-BI mediates uptake of cholesteryl esters from HDL for the synthesis of glucocorticoid hormones such as cortisol (3-5). On platelets, HDL binding to surface SR-BI inhibits aggregation and increases platelet survival time (3-5). SR-BI and its SR-BII isoform also bind bacterial lipopolysaccharides, facilitating uptake of various bacteria by cells such as peritoneal macrophages (7, 8). This uptake enhances inflammatory responses which, unless properly controlled, can result in sepsis (8-10).

1. Calvo, D. and M. A. Vega (1993) J. Biol. Chem. 268:18929.
2. Webb, N.R. et al. (1998) J. Biol. Chem. 273:15241.
3. Chadwick, A.C. and D. Sahoo (2013) Curr. Opin. Endocrinol. Diabetes Obes. 20:124.
4. Hoekstra, M. et al. (2012) Curr. Opin. Lipidol. 23:127.
5. Vergeer, M. et al. (2011) N. Engl. J. Med. 364:136.
6. Guo, L. et al. (2011) J. Lipid Res. 52:2272.
7. Vishnyakova, T.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16888.
8. Baranova, I.N. et al. (2012) J. Immunol. 188:1371.
9. Leelahavanichkul, A. et al. (2012) J. Immunol. 188:2749.
10. Guo, L. et al. (2009) J. Biol. Chem. 284:19826.    

Bioinformatics

• Uniprot:
  • Q61009-1()