Recombinant Mouse SPARC Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SPARC Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells. Schiemann, B.J. et al. (2003) Mol. Biol. Cell. 14:3977. The ED 50 for this effect is 0.6-2.4 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse SPARC protein Ala18-Ile302, with a C-terminal 10-His tag
• Accession #: P07214
• N-terminal Sequence: Ala18
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sparc
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SPARC Protein, CF

• Basement-membrane protein 40
• BM-40
• ONcysteine-rich protein
• Osteonectin
• Secreted protein acidic and rich in cysteine
• secreted protein, acidic, cysteine-rich (osteonectin)
• SPARC

Background

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1 - 5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1 - 5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3 - 5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3 - 5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92% and 83% aa identity with rat, human, dog, cow and chick SPARC, respectively.

1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.

Publications for SPARC (942-SP)(6)

Publications using 942-SP

• L Hu, F He, M Huang, Q Zhao, L Cheng, N Said, Z Zhou, F Liu, YS Dai SPARC promotes insulin secretion through down-regulation of RGS4 protein in pancreatic &amp;beta cells Sci Rep, 2020-10-16;10(1):17581. 2020-10-16 [PMID: 33067534] (Cell Culture, Mouse)
• B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860] (Bioassay, Mouse)
• EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury Front Cell Neurosci, 2018-02-01;12(0):22. 2018-02-01 [PMID: 29449802] (Bioassay, Mouse)
• A Melouane, A Carbonell, M Yoshioka, J Puymirat, J St-Amand Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells PLoS ONE, 2018-02-08;13(2):e0192714. 2018-02-08 [PMID: 29420632] (Bioassay, Mouse)
• Luo Z, Zhou Y, Luo P, Zhao Q, Xiao N, Yu Y, Yan Q, Lu G, Cheng L SPARC deficiency affects bone marrow stromal function, resulting in impaired B lymphopoiesis. J Leukoc Biol, 2014-03-05;96(1):73-82. 2014-03-05 [PMID: 24598056] (Bioassay, Mouse)
• Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions. J. Neurosci., 2011-03-16;31(11):4154-65. 2011-03-16 [PMID: 21411656] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Sparc
• Uniprot:
  • P07214()