Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein, CF

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1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus (Catalog # 461-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.6‑3 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.6-3 µg/mL.
Source
E. coli-derived mouse Sonic Hedgehog/Shh protein
Cys25-Gly198, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Cys25
Protein/Peptide Type
Recombinant Proteins
Gene
Shh
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
461-SH/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein, CF

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Echelard, Y. et al. (1993) Cell 75:1417.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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Publications for Sonic Hedgehog/Shh (461-SH/CF)(25)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Xenopus.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(19)
In Vivo
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(13)
Rat
(6)
Avian - Quail
(1)
Chicken
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using 461-SH/CF Applications Species
D Gu, S Wang, S Zhang, P Zhang, G Zhou Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine Mol Med Rep, 2017;0(0):. 2017 [PMID: 28983586] (Bioassay, Rat) Bioassay Rat
Lan T, Kisseleva T, Brenner D Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation. PLoS ONE, 2015;10(7):e0129743. 2015 [PMID: 26222337] (Bioassay, Mouse) Bioassay Mouse
Chavez M, Ena S, Van Sande J, de Kerchove d'Exaerde A, Schurmans S, Schiffmann S Modulation of Ciliary Phosphoinositide Content Regulates Trafficking and Sonic Hedgehog Signaling Output. Dev Cell, 2015;34(3):338-50. 2015 [PMID: 26190144] (Bioassay, Mouse) Bioassay Mouse
Yuan X, Yang S Deletion of IFT80 Impairs Epiphyseal and Articular Cartilage Formation Due to Disruption of Chondrocyte Differentiation. PLoS ONE, 2015;10(6):e0130618. 2015 [PMID: 26098911] (Bioassay, Mouse) Bioassay Mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015;10(1):e0117908. 2015 [PMID: 25629162] (Bioassay, Mouse) Bioassay Mouse
Takenaka-Ninagawa, Nana, Kawabata, Yuka, Watanabe, Shogo, Nagata, Kohzo, Torihashi, Shigeko Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome. PLoS ONE, 2014;9(8):e104462. 2014 [PMID: 25111735] (Bioassay, Rat) Bioassay Rat
Rozycki M, Lodyga M, Lam J, Miranda M, Fatyol K, Speight P, Kapus A The fate of the primary cilium during myofibroblast transition. Mol Biol Cell, 2014;25(5):643-57. 2014 [PMID: 24403605] (Bioassay, Human) Bioassay Human
Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells. Neuron, 2013;80(5):1145-58. 2013 [PMID: 24314726] (Bioassay, Mouse) Bioassay Mouse
Engevik A, Feng R, Yang L, Zavros Y The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair. Endocrinology, 2013;154(12):4627-39. 2013 [PMID: 24092639] (Mouse) Mouse
Liu, Xian Shu, Chopp, Michael, Wang, Xin Li, Zhang, Li, Hozeska-Solgot, Ann, Tang, Tao, Kassis, Haifa, Zhang, Rui Lan, Chen, Charles, Xu, Jennifer, Zhang, Zheng Ga MicroRNA-17-92 cluster mediates the proliferation and survival of neural progenitor cells after stroke. J Biol Chem, 2013;288(18):12478-88. 2013 [PMID: 23511639] (In Vivo, Mouse) In Vivo Mouse
Show All 25 Publications.

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Bioinformatics

Gene Symbol Shh
Entrez
Uniprot