Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of ICAM-3 expressing CHO Chinese hamster ovary cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse SIGNR1/CD209b Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 30-50% will adhere after 1 hour at room temperature.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse SIGNR1/CD209b protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Mouse SIGNR1 (Ser76-Gly325) Accession # Q8CJ91
  N-terminus			C-terminus

• Accession #: Q8CJ91
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd209b
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 55.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SIGNR1/CD209b Fc Chimera Protein, CF

• CD209 antigen like protein B
• CD209b
• DC SIGN related protein 1
• DC SIGNR1
• OtB7
• SIGNR1

Background

SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta  and gamma  isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC-SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11-13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).

1. Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
2. Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
3. McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
4. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
5. Parent, S.A. et al. (2002) Gene 293:33.
6. Kang, Y.-S. et al. (2003) Int. Immunol. 15:177.
7. Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327.
8. Galustian, C. et al. (2003) Int. Immunol. 16:853.
9. Taylor, P.R. et al. (2004) J. Immunol. 172:1157.
10. Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
11. Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962.
12. Lanoue, A. et al. (2004) J. Exp. Med. 200:1383.
13. Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
14. Nagaoka, K. et al. (2005) Int. Immunol. 17:827.
15. Takahara, K. et al. (2003) Int. Immunol. 16:819.

Publications for SIGNR1/CD209b (1836-SR)(1)

Publication using 1836-SR

• T Uto, T Fukaya, H Takagi, K Arimura, T Nakamura, N Kojima, B Malissen, K Sato Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity Nat Commun, 2016-04-12;7(0):11273. 2016-04-12 [PMID: 27068492] (Enzyme Assay, Human)

Bioinformatics

• Gene Symbol: Cd209b
• Uniprot:
  • Q8CJ91()