Recombinant Mouse SEZ6L Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse SEZ6L Protein, CF Summary

• Details of Functionality: Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells. 2 μg/mL of Recombinant Mouse SEZ6L is able to signifcantly induce cell clumping and inhibit neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse SEZ6L protein
  Glu32-Ser889, with a C-terminal 6-His tag
• Accession #: NP_064366
• N-terminal Sequence: Glu32
• Protein/Peptide Type: Recombinant Proteins
• Gene: Sez6l
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 115-185 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse SEZ6L Protein, CF

• Acig1
• AIG1
• Aig1L
• BSRP-B
• seizure related 6 homolog (mouse)-like
• seizure related gene 6-like
• SEZ6L

Background

Seizure 6-like Protein (SEZ6L), also known as Brain-specific Receptor-like Protein B (BSRP-B), is a member of the SEZ6 family of proteins with a molecular weight of approximately 200 kDa (1). Mouse SEZ6L is a type I transmembrane glycoprotein composed of an extracellular domain (ECD) that contains three CUB domains and five short consensus repeats (SCRs) (1). Alternative splicing generates an isoform that contains a phenylalanine substitution for amino acids (aa) 807-872 of the fifth SCR domain (1). Mouse SEZ6L shares 80% and 97% aa sequence identity with the human and rat orthologs, respectively. It is expressed in the plasma membrane and endoplasmic reticulum of neurons and lung bronchiolar epithelium and likely regulates PKC alpha activation (1, 2). In mouse pancreatic beta cells, SEZ6L has been shown to be a substrate for BACE-2, which cleaves its ECD at a site 16 aa upstream from its transmembrane domain (3). Additionally, human SEZ6L has been shown to be involved in the maintenance of cellular cholesterol homeostasis (4). BSRP-C also known as SEZ6, has been reported to inhibit neurite outgrowth in mice (5). R&D Systems in-house testing shows that mouse SEZ6L, also exhibits this function. Genetic variations in human Sez6l have been associated with bipolar disorder I and an increased risk for lung cancer (2, 6, 7).

1. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
2. Nishioka, M. et al. (2000) Oncogene 19:6251.
3. Stützer, I. et al. (2013) J. Biol. Chem. 288:10536.
4. Blattmann, P. et al. (2013) PLoS Genetics 9:e1003338.
5. Gunnersen, J.M. et al. (2007) Neuron 56:621.
6. Gorlov, I.P. et al. (2007) Cancer Res. 67:8406.
7. Xu, C. et al. (2013) J. Affect. Disord. 145:95.

Bioinformatics

• Gene Symbol: Sez6l
• Uniprot:
  • NP_064366()