Recombinant Mouse Semaphorin 6A Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Semaphorin 6A Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED₅₀ for this effect is 0.2-1.2 μg/mL.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse Semaphorin 6A protein
  

  Mouse Sema 6A (Gly19-Thr649) Accession # O35464	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O35464
• N-terminal Sequence: Gly19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 94-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Semaphorin 6A Fc Chimera Protein, CF

• KIAA1368HT018
• sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
• Sema VIA
• SEMA
• Sema6A
• SEMA6A1
• SEMA6A-1
• Semaphorin 6A
• semaphorin 6A-1
• Semaphorin VIA
• semaphorin-6A
• semaphorin-6A-1
• SEMAQ
• VIA

Background

Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta propeller N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, mouse Sema6A shares 94% and 98% aa sequence identity with human and rat Sema6A, respectively. Alternative splicing generates additional isoforms with a 26 aa deletion within the sema domain or a 55 aa deletion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4 (3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).

1. Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292.
2. Zhou, L. et al. (1997) Mol. Cell. Neurosci. 9:26.
3. Suto, F. et al. (2005) J. Neurosci. 25:3628.
4. Matsuoka, R.L. et al. (2011) Nature 470:259.
5. Runker, A.E. et al. (2008) Neural Dev. 3:34.
6. Suto, F. et al. (2007) Neuron 53:535.
7. Haklai-Topper, L. et al. (2010) EMBO J. 29:2635.
8. Xu, X.M. et al. (2000) J. Neurosci. 20:2638.
9. Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516.
10. Mauti, O. et al. (2007) Neural Dev. 2:28.
11. Segarra, M. et al. (2012) Blood 120:4104.
12. Kigel, B. et al. (2011) Blood 118:4285.
13. Bernard, F. et al. (2012) Glia 60:1590.
14. Gautier, G. et al. (2006) Am. J. Pathol. 168:453.

Publications for Semaphorin 6A (9017-S6)(1)

Publication using 9017-S6

• J Kaiser, M Maibach, I Salpeter, N Hagenbuch, VBC de Souza, MD Robinson, ME Schwab The Spinal Transcriptome after Cortical Stroke: In Search of Molecular Factors Regulating Spontaneous Recovery in the Spinal Cord J. Neurosci., 2019-04-08;39(24):4714-4726. 2019-04-08 [PMID: 30962276] (Bioassay, Rat)

Bioinformatics

• Uniprot:
  • O35464()