Recombinant Mouse Reg3B Protein, CF

Recombinant Mouse Reg3B stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.2-1 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Reg3B Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is typically 0.2-1 μg/mL.
• Source: E. coli-derived mouse Reg3B protein
  Glu27-Gly175, with an N-terminal Met
• Accession #: P35230
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Reg3b
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Reg3B Protein, CF

• PAP1
• Reg3B

Background

Regenerating islet-derived protein 3-beta (Reg3B), also known as Reg2, pancreatitis-associated protein 1 (PAP1), hepatocarcinoma-intestine-pancreas (HIP), and peptide 23, is one of several secreted Reg proteins that contain a single C-type lectin domain and contribute to anti-microbial immunity in the gut (1, 2). Reg3B is secreted as a 149 amino acid (aa) protein that contains a conserved protease-sensitive site which allows removal of an 11 residue propeptide from the N-terminus (3). In Reg3G, this short propeptide serves as an inhibitor that maintains the proprotein in an inactive state (3). Mature mouse Reg3B shares 92% aa sequence identity with rat Reg3B. Reg3B is expressed by neuroendocrine and Paneth cells in the small intestine, acinar and islet beta cells in the pancreas, and developing motor and sensory neurons in the CNS (4, 5). It is up-regulated in regenerating neurons, and it promotes Schwann cell proliferation (6). It is required for the ability of CNTF to support the survival of injured neurons (5). Reg3B is also upregulated in pancreatitis and can protect mice against inflammation and streptozotocin induced beta cell death (7, 8). In the gut, Reg3B is overexpressed in colitis and inflammatory bowel disease and by exposure to pathogenic bacteria (9-12). Its expression is dependent on the activation of TLR2 (10). Reg3B acts to clear bacterial infections in vivo and provide host resistance to infection (10, 12). It binds to the Lipid A moiety of LPS on Gram negative bacteria, and this interaction is important for its bactericidal activity (11, 13, 14). On S. typhimurium, the LPS saccharide O-antigen prevents the binding of Reg3B and thereby protects those cells from Reg3B induced killing (11, 14). In contrast to its tissue-protective functions, Reg3B enhances tumor growth and angiogenesis and inhibits tumor macrophage and T cell mediated inflammation (15). It also exacerbates the hepatotoxicity of acetominophen by promoting the nitration of proteins in the liver (16).

1. Parikh, A. et al. (2012) Biomol. Concepts 3:57.
2. Narushima, Y. et al. (1997) Gene 185:159.
3. Mukherjee, S. et al. (2009) J. Biol. Chem. 284:4881.
4. Lasserre, C. et al. (1999) Am. J. Pathol. 154:1601.
5. Tebar, L.A. et al. (2008) Proc. Natl. Acad. Sci. USA 105:11400.
6. Livesey, F.J. et al. (1997) Nature 390:614.
7. Vasseur, S. et al. (2004) J. Biol. Chem. 279:7199.
8. Xiong, X. et al. (2011) Am. J. Physiol. Endocrinol. Metab. 300:E669.
9. Ogawa, H. et al. (2003) Inflamm. Bowel Dis. 9:162.
10. Dessein, R. et al. (2009) Gut 58:771.
11. Stelter, C. et al. (2011) PLoS ONE 6:e20749.
12. van Ampting, M.T.J. et al. (2012) Infect. Immun. 80:1115.
13. Miki, T. et al. (2012) J. Biol. Chem. 287:34844.
14. Miki, T. and W-D. Hardt (2013) PLoS ONE 7:e69901.
15. Gironella, M. et al. (2013) Cancer Res. 73:5682.
16. Yun, J.W. et al. (2013) Free Radic. Biol. Med. 65C:291.

Publications for Reg3B (5110-RG)(1)

Publication using 5110-RG

• FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang Helicobacter pylori-induced Rev-erb&amp;alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense Cellular and Molecular Gastroenterology and Hepatology, 2021-03-03;0(0):. 2021-03-03 [PMID: 33676046] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Reg3b
• Uniprot:
  • P35230()