Recombinant Mouse RAGE Fc Chimera Protein, CF

Images

 
There are currently no images for AGER (1179-RG).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse RAGE Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. rmRAGE/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody-coated plate (0.5 µg/well) can bind biotinylated advanced glycation endproducts of bovine serum albumin (AGE-BSA, Catalog # BT4127) with a linear range of 0.02-1 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse RAGE protein
Mouse RAGE
(Gln24 - Ala342)
Accession #O35444
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Ager
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
60.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-85 kDa, reducing conditions
Publications
Read Publications using
1179-RG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse RAGE Fc Chimera Protein, CF

  • advanced glycosylation end product-specific receptor
  • AGER
  • RAGE isoform delta
  • RAGE isoform sRAGE-delta
  • RAGE
  • Receptor for advanced glycosylation end products
  • receptor for advanced glycosylation end-products
  • SCARJ1

Background

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.

Customers Who Viewed This Item Also Viewed...

NB100-2322
Species: Hu, Mu, Rt, Bv, Ca, Rb, Sh
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP1-80851
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-45224
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
7270-IL/CF
Species: Hu
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-29403
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt, Mk
Applications: WB, DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P
NB100-56722
Species: Hu, Mu, Rt, Ca
Applications: WB, DB, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
AF1052
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
NBP2-03950
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
AF3059
Species: Mu
Applications: WB, Simple Western, ICC

Publications for AGER (1179-RG)(9)

We have publications tested in 3 confirmed species: Mouse, Rat, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, ELISA Standard, Flow Cytometry, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(3)
ELISA Standard
(1)
Flow Cytometry
(1)
In Vivo
(3)
All Applications
Filter By Species
Mouse
(6)
Rat
(1)
N/A
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using 1179-RG Applications Species
F Zhang, X Su, G Huang, XF Xin, EH Cao, Y Shi, Y Song sRAGE alleviates neutrophilic asthma by blocking HMGB1/RAGE signalling in airway dendritic cells Sci Rep, 2017;7(1):14268. 2017 [PMID: 29079726] (In Vivo, Mouse) In Vivo Mouse
R Blondonnet, J Audard, C Belville, G Clairefond, J Lutz, D Bouvier, L Roszyk, C Gross, M Lavergne, M Fournet, L Blanchon, C Vachias, C Damon-Soub, V Sapin, JM Constantin, M Jabaudon RAGE inhibition reduces acute lung injury in mice Sci Rep, 2017;7(1):7208. 2017 [PMID: 28775380] (In Vivo, Mouse) In Vivo Mouse
Y Gao, M Bielohuby, T Fleming, GF Grabner, E Foppen, W Bernhard, M Guzmán-Rui, C Layritz, B Legutko, E Zinser, C García-Các, RM Buijs, SC Woods, A Kalsbeek, RJ Seeley, PP Nawroth, M Bidlingmai, MH Tschöp, CX Yi Dietary sugars, not lipids, drive hypothalamic inflammation Mol Metab, 2017;6(8):897-908. 2017 [PMID: 28752053] (Bioassay, N/A) Bioassay N/A
A Braley, T Kwak, J Jules, E Harja, R Landgraf, BI Hudson Regulation of RAGE ectodomain shedding and its role in cell function J Biol Chem, 2016;0(0):. 2016 [PMID: 27022018] (Bioassay, Rat) Bioassay Rat
Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages. PLoS ONE, 2015;10(4):e0123293. 2015 [PMID: 25849867] (Binding Assay, Mouse) Binding Assay Mouse
Mizumoto S, Takahashi J, Sugahara K Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells. J. Biol. Chem., 2012;287(23):18985-94. 2012 [PMID: 22493510] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Kuhla A, Hettwer C, Menger MD Oxidative stress-associated rise of hepatic protein glycation increases inflammatory liver injury in uncoupling protein-2 deficient mice. Lab. Invest., 2010;90(8):1189-98. 2010 [PMID: 20368701] (In Vivo, Mouse) In Vivo Mouse
Su X, Looney MR, Gupta N, Matthay MA Receptor for advanced glycation end-products (RAGE) is an indicator of direct lung injury in models of experimental lung injury. Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(1):L1-5. 2009 [PMID: 19411309] (ELISA Standard, N/A) ELISA Standard N/A
Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock. Nat. Med., 2007;13(9):1042-9. 2007 [PMID: 17767165] (Bioassay, Mouse) Bioassay Mouse

Reviews for AGER (1179-RG) (0)

There are no reviews for AGER (1179-RG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for AGER (1179-RG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse RAGE Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Ager
Uniprot