Recombinant Mouse Persephin Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse Persephin Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TT human medullary thyroid cancer cells. The ED50 for this effect is 1-3 ng/mL.
Source
E. coli-derived mouse Persephin protein
Ala61-Gly156
Accession #
N-terminal Sequence
Ala61
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Pspn
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.3 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
2479-PS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose and with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCI containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Persephin Protein

  • Persephin
  • PSP
  • PSPN

Background

Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38 - 46% amino acid identity with family members GDNF, neurturin and artemin. Persephin is expressed at very low levels in most tissues (1). The 10 - 12 kDa mature protein contains several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked homodimer. Mature mouse persephin shares 94%, 81%, 79% and 76% amino acid sequence identity with rat, human, bovine and canine persephin, respectively. Like other GDNF family members, persephin acts through engagement of a glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney development (1). These effects are correlated with the expression patterns of its specific receptor, GFR alpha 4, and RET (2, 3). Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system and include GPI-linked, transmembrane and soluble forms (3, 4). In vitro, persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET (2, 5). This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members (6). Disruption of the persephin gene results in mice that are morphologically normal but have more damage and less effective repair after a central nervous system insult simulating a stroke. Microinjection of persephin prior to treatment protects against damage in both wild-type and mutant mouse brains, but surprisingly, high doses of persephin are detrimental (7).

  1. Milbrandt, J. et al. (1998) Neuron 20:245.
  2. Lindahl, M. et al. (2001) J. Biol. Chem. 276:9344.
  3. Lindahl, M. et al. (2000) Mol. Cell. Neurosci. 15:522.
  4. Akerud, P. et al. (2002) Mol. Cell. Neurosci. 21:205.
  5. Enokido, Y. et al. (1998) Curr. Biol. 8:1019.
  6. Yang, J. et al. (2004) FEBS Lett. 569:267.
  7. Tomac, A. C. et al. (2002) Proc. Natl. Acad. Sci. USA 99:9521.

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Publications for Persephin (2479-PS)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Pspn
Uniprot